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Catalog
E044215
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species
Mouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse anti-GPC antibody. No significant cross-reactivity or interference between mouse anti-GPC antibody and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse anti-GPC antibody is typically less than 0.15 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determ
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Intended UseMouse GT1b ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GPC. Standards and samples are pipetted into the wells with a biotin-conjugated GPC and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of anti-GPC antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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