Mouse Gnrhr ELISA Kit

Alternative name
Mouse gnRH receptor ELISA Kit;Mouse GnRH-R ELISA Kit;Mouse gonadotropin releasing hormone receptor ELISA Kit;Mouse gonadotropin-releasing hormone receptor ELISA Kit;
Catalog
E044232
species
Mouse
GeneGnrhr
SpecificityThis assay has high sensitivity and excellent specificity for detection of GNRHR-Ab. No significant cross-reactivity or interference between GNRHR-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GNRHR-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Gnrhr ELISA Kit allows for the in vitro quantitative determination of Gnrhr , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalIntended Uses: This GNRHR-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GNRHR-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GNRHR-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GNRHR-Ab antibody and an GNRHR-Ab-HRP conjugate. The assay sample and buffer are incubated together with GNRHR-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNRHR-Ab concentration since GNRHR-Ab from samples and GNRHR-Ab-HRP conjugate compete for the anti-GNRHR-Ab antibody binding site. Since the number of sites is limited, as more sites are occupied by GNRHR-Ab from the sample, fewer sites are left to bind GNRHR-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNRHR-Ab concentration in each sample is interpolated from this standard curve.
Mouse Gonadotropin-releasing hormone receptor Protein information
Uniprot ID GNRHR_MOUSE
Uniprot AC Q01776; Q61611;
UniGene Mm.26791;
GeneID 14715
KEGG mmu:14715;
Mouse Gonadotropin-releasing hormone receptor Protein SEQUENCE
SEQUENCE 327 AA; 37684 MW; FDDC7B985306FC9F CRC64;
MANNASLEQD PNHCSAINNS IPLIQGKLPT LTVSGKIRVT VTFFLFLLST
AFNASFLLKL QKWTQKRKKG KKLSRMKVLL KHLTLANLLE TLIVMPLDGM
WNITVQWYAG EFLCKVLSYL KLFSMYAPAF MMVVISLDRS LAITQPLAVQ
SNSKLEQSMI SLAWILSIVF AGPQLYIFRM IYLADGSGPT VFSQCVTHCS
FPQWWHQAFY NFFTFGCLFI IPLLIMLICN AKIIFALTRV LHQDPRKLQL
NQSKNNIPRA RLRTLKMTVA FATSFVVCWT PYYVLGIWYW FDPEMLNRVS
EPVNHFFFLF AFLNPCFDPL IYGYFSL
UCSC uc008xxl.1; mouse.;

Category

Mouse Gnrhr ELISA Kit

Mouse gnRH receptor ELISA Kit;Mouse GnRH-R ELISA Kit;Mouse gonadotropin releasing hormone receptor ELISA Kit;Mouse gonadotropin-releasing hormone receptor ELISA Kit;