Mouse Musk ELISA Kit

Alternative name
Mouse muscle-specific tyrosine-protein kinase receptor ELISA Kit;Mouse MuSK ELISA Kit;Mouse muscle-specific kinase receptor ELISA Kit;Mouse Nsk2 ELISA Kit;Mouse Mdk4 ELISA Kit;Mouse Mlk ELISA Kit;Mouse Nsk1 ELISA Kit;Mouse Nsk3 ELISA Kit;Mouse muscle, skeletal, receptor tyrosine kinase ELISA Kit;Mouse muscle, skeletal receptor tyrosine-protein kinase ELISA Kit;Mouse muscle localized kinase 2 ELISA Kit;Mouse muscle-specific protein kinase secretory isoform ELISA Kit;
Catalog
E044288
species
Mouse
GeneMusk
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Musk ELISA Kit allows for the in vitro quantitative determination of Musk , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Anti-MUSK ELISA kit applies the competitive enzyme immunoassay technique utilizing MUSK antigen and an Anti-MUSK-HRP conjugate. The assay sample and buffer are incubated together with Anti-MUSK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-MUSK concentration since Anti-MUSK from samples and Anti-MUSK-HRP conjugate compete for the MUSK antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-MUSK from the sample, fewer sites are left to bind Anti-MUSK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-MUSK concentration in each sample is interpolated from this standard curve.
Mouse Muscle, skeletal receptor tyrosine-protein kinase Protein information
Uniprot ID MUSK_MOUSE
Uniprot AC Q61006; Q61005; Q61987; Q61988;
UniGene Mm.16148;
GeneID 18198
KEGG mmu:18198;
Mouse Muscle, skeletal receptor tyrosine-protein kinase Protein SEQUENCE
SEQUENCE 868 AA; 96693 MW; AFE4E644C6869933 CRC64;
MRELVNIPLL QMLTLVAFSG TEKLPKAPVI TTPLETVDAL VEEVATFMCA
VESYPQPEIS WTRNKILIKL FDTRYSIREN GQLLTILSVE DSDDGIYCCI
ANNGVGGAVE SCGALQVKMK PKITRPPINV KIIEGLKAVL PCTTMGNPKP
SVSWIKGDNA LRENSRIAVL ESGSLRIHNV QKEDAGQYRC VAKNSLGTAY
SKLVKLEVEV FARILRAPES HNVTFGSFVT LRCTAIGIPV PTISWIENGN
AVSSGSIQES VKDRVIDSRL QLFITKPGLY TCIATNKHGE KFSTAKAAAT
VSIAEWSKSQ KDSQGYCAQY RGEVCDAVLA KDALVFFNTS YRDPEDAQEL
LIHTAWNELK AVSPLCRPAA EALLCNHLFQ ECSPGVVPTP MPICREYCLA
VKELFCAKEW QAMEGKAHRG LYRSGMHLLP VPECSKLPSM HRDPTACTRL
PYLDYKKENI TTFPSITSSR PSADIPNLPA STSSFAVSPA YSMTVIISIV
SSFALFALLT IATLYCCRRR KEWKNKKRES TAVTLTTLPS ELLLDRLHPN
PMYQRMPLLL NPKLLSLEYP RNNIEYVRDI GEGAFGRVFQ ARAPGLLPYE
PFTMVAVKML KEEASADMQA DFQREAALMA EFDNPNIVKL LGVCAVGKPM
CLLFEYMAYG DLNEFLRSMS PHTVCSLSHS DLSTRARVSS PGPPPLSCAE
QLCIARQVAA GMAYLSERKF VHRDLATRNC LVGETMVVKI ADFGLSRNIY
SADYYKADGN DAIPIRWMPP ESIFYNRYTT ESDVWAYGVV LWEIFSYGLQ
PYYGMAHEEV IYYVRDGNIL ACPENCPLEL YNLMRLCWSK LPADRPSFCS
IHRILQRMCE RAEGTVGV
UCSC uc033icg.1; mouse. [Q61006-1];

Category

Mouse Musk ELISA Kit