Mouse Mog ELISA Kit

Alternative name
Mouse B230317G11Rik ELISA Kit;Mouse myelin oligodendrocyte glycoprotein ELISA Kit;Mouse myelin-oligodendrocyte glycoprotein ELISA Kit;
Catalog
E044294
species
Mouse
GeneMog
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Mog ELISA Kit allows for the in vitro quantitative determination of Mog , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: Anti-MOG ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti-Myelin Oligodendrocyte Glycoprotein antigen and an Anti-MOG-HRP conjugate. The assay sample and buffer are incubated together with Anti-MOG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-MOG concentration since Anti-MOG from samples and Anti-MOG-HRP conjugate compete for the Anti-Myelin Oligodendrocyte Glycoprotein antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-MOG from the sample, fewer sites are left to bind Anti-MOG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-MOG concentration in each sample is interpolated from this standard curve.
Mouse Myelin-oligodendrocyte glycoprotein Protein information
Uniprot ID MOG_MOUSE
Uniprot AC Q61885; P70364; Q62003;
UniGene Mm.210857;
GeneID 17441
KEGG mmu:17441;
Mouse Myelin-oligodendrocyte glycoprotein Protein SEQUENCE
SEQUENCE 246 AA; 28271 MW; 1F1A8A4A0D5CFB89 CRC64;
MACLWSFSWP SCFLSLLLLL LQLSCSYAGQ FRVIGPGYPI RALVGDEAEL
PCRISPGKNA TGMEVGWYRS PFSRVVHLYR NGKDQDAEQA PEYRGRTELL
KETISEGKVT LRIQNVRFSD EGGYTCFFRD HSYQEEAAME LKVEDPFYWV
NPGVLTLIAL VPTILLQVPV GLVFLFLQHR LRGKLRAEVE NLHRTFDPHF
LRVPCWKITL FVIVPVLGPL VALIICYNWL HRRLAGQFLE ELRNPF

Category

Mouse Mog ELISA Kit

Mouse B230317G11Rik ELISA Kit;Mouse myelin oligodendrocyte glycoprotein ELISA Kit;Mouse myelin-oligodendrocyte glycoprotein ELISA Kit;