Mouse Pnma2 ELISA Kit

Alternative name
Mouse A830049P17Rik ELISA Kit;Mouse mKIAA0883 ELISA Kit;Mouse paraneoplastic antigen MA2 ELISA Kit;Mouse paraneoplastic antigen Ma2 homolog ELISA Kit;
Catalog
E044330
species
Mouse
GenePnma2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Pnma2 ELISA Kit allows for the in vitro quantitative determination of Pnma2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the Assay: Anti-PNMA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with paraneoplastic Ma antigen 2. Standards or samples are then added to the microtiter plate wells and Anti-PNMA2 if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of Anti-PNMA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated paraneoplastic Ma antigen 2 is added to each well to "sandwich" the Anti-PNMA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-PNMA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-PNMA2 concentration in each sample is interpolated from this standard curve.
Mouse Paraneoplastic antigen Ma2 homolog Protein information
Uniprot ID PNMA2_MOUSE
Uniprot AC Q8BHK0;
UniGene Mm.44358;
GeneID 239157
KEGG mmu:239157;
Mouse Paraneoplastic antigen Ma2 homolog Protein SEQUENCE
SEQUENCE 365 AA; 41201 MW; 0F90C940B9D843D9 CRC64;
MAVALLEEWC KIMGVDVQKS LLVVDIPVDC GEPEIQTVLQ EALKCVGSYR
LLGKIFQKQD NTSVVLVELM EDTDMSVVPS EVQGKGGVWK VIFKTPNQDT
EFLQRLNLFL EKEGQTVAGM FRALKHEGVS PATPPCTSPE LLAHLTGQAM
VHGQRPLLPV KYCKMRIFSG STAAAPEEEP FEVWLEQATE IAKEWPIPEA
EKKRWVAESL RGPALDLMHI VQADNPSISV GECLEAFKQV FGSTESRRTS
QVKYLRTYQQ EGEKISAYVL RLETLLRRAV EKRAIPRNIA DQVRLEQVMA
GANLGNVLWC RLQELKDQGP LPTFLQLMKV IREEEEEEDA YFEQESREEP
GEREGSGCWN NSRNN
UCSC uc007ukq.2; mouse.;

Category

Mouse Pnma2 ELISA Kit

Mouse A830049P17Rik ELISA Kit;Mouse mKIAA0883 ELISA Kit;Mouse paraneoplastic antigen MA2 ELISA Kit;Mouse paraneoplastic antigen Ma2 homolog ELISA Kit;