Mouse Sla ELISA Kit

Alternative name
Mouse src-like-adapter protein 1 ELISA Kit;Mouse Slap-1 ELISA Kit;Mouse mSLAP ELISA Kit;Mouse Slap ELISA Kit;Mouse Slap1 ELISA Kit;Mouse src-like adaptor ELISA Kit;Mouse src-like-adapter ELISA Kit;
Catalog
E044382
species
Mouse
GeneSla
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Sla ELISA Kit allows for the in vitro quantitative determination of Sla , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: ASLAg ELISA kit applies the competitive enzyme immunoassay technique utilizing soluble Liver Antigen and an ASLAg-HRP conjugate. The assay sample and buffer are incubated together with ASLAg-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASLAg concentration since ASLAg from samples and ASLAg-HRP conjugate compete for the soluble Liver Antigen binding site. Since the number of sites is limited, as more sites are occupied by ASLAg from the sample, fewer sites are left to bind ASLAg-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASLAg concentration in each sample is interpolated from this standard curve.
Mouse Src-like-adapter Protein information
Uniprot ID SLAP1_MOUSE
Uniprot AC Q60898; Q8C9Q8; Q8CAT0; Q8CBE9; Q8QZX8;
UniGene Mm.7601;
GeneID 20491
KEGG mmu:20491;
Mouse Src-like-adapter Protein SEQUENCE
SEQUENCE 281 AA; 31681 MW; B347921656A74DA1 CRC64;
MGNSMKSTSP PSERPLSSSE GLESDFLAVL TDYPSPDISP PIFRRGEKLR
VISDEGGWWK AISLSTGRES YIPGICVARV YHGWLFEGLG RDKAEELLQL
PDTKIGSFMI RESETKKGFY SLSVRHRQVK HYRIFRLPNN WYYISPRLTF
QCLEDLVTHY SEVADGLCCV LTTPCLAQNI PAPTSHPSPC TSPGSPVTLR
QKTFDWKRVS RLQEGSEGAE NPLRVDESLF SYGLRESIAS YLSLTGDDSS
SFDRKKKSLS LMYTGSKRKS SFFSAPQYFE D
UCSC uc007war.2; mouse. [Q60898-1];

Category

Mouse Sla ELISA Kit

Mouse src-like-adapter protein 1 ELISA Kit;Mouse Slap-1 ELISA Kit;Mouse mSLAP ELISA Kit;Mouse Slap ELISA Kit;Mouse Slap1 ELISA Kit;Mouse src-like adaptor ELISA Kit;Mouse src-like-adapter ELISA Kit;