-
Alternative name
Mouse APJ endogenous ligand ELISA Kit;Mouse Apel ELISA Kit;Mouse 6030430G11Rik ELISA Kit;Mouse apelin ELISA Kit;Mouse preproapelin ELISA Kit;
-
Catalog
E044501
-
species
Mouse
-
GeneApln
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse APLN. No significant cross-reactivity or interference between Mouse APLN and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity7.8 pg/ml
-
Intended UseMouse Apln ELISA Kit allows for the in vitro quantitative determination of Apln , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for apelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any apelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for apelin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of apelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
-
Mouse Apelin-13 Protein information
-
Uniprot ID
APEL_MOUSE
-
Uniprot AC
Q9R0R4;
-
UniGene
Mm.29262;
-
GeneID
30878
-
KEGG
mmu:30878;
-
Mouse Apelin-13 Protein SEQUENCE
-
SEQUENCE 77 AA; 8658 MW; D71E0D58E47D2376 CRC64;
MNLRLCVQAL LLLWLSLTAV CGVPLMLPPD GTGLEEGSMR YLVKPRTSRT
GPGAWQGGRR
KFRRQRPRLS HKGPMPF
-
UCSC
uc009tbu.1; mouse.;
-