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Alternative name
Mouse Apo-AII ELISA Kit;Mouse ApoA-II ELISA Kit;Mouse apolipoprotein A2 ELISA Kit;Mouse ProapoA-II ELISA Kit;Mouse Alp-2 ELISA Kit;Mouse ApoAII ELISA Kit;Mouse Apoa-2 ELISA Kit;Mouse Hdl-1 ELISA Kit;Mouse apolipoprotein A-II ELISA Kit;
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Catalog
E044528
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species
Mouse
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GeneApoa2
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SpecificityThis kit recognizes natural and recombinant Mouse ApoA2. No significant cross-reactivity or interference between Mouse ApoA2 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of Mouse ApoA2 is 4.688pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
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Intended UseMouse Apoa2 ELISA Kit allows for the in vitro quantitative determination of Apoa2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoA2. You can calculate the concentration of ApoA2 in the samples by comparing the OD of the samples to the standard curve.
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Mouse Proapolipoprotein A-II Protein information
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Uniprot ID
APOA2_MOUSE
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Uniprot AC
P09813; Q3UKX6; Q61317;
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UniGene
Mm.389209;
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GeneID
11807
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KEGG
mmu:11807;
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Mouse Proapolipoprotein A-II Protein SEQUENCE
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SEQUENCE 102 AA; 11309 MW; F50142E0D118665B CRC64;
MKLLAMVALL VTICSLEGAL VKRQADGPDM QSLFTQYFQS MTDYGKDLME
KAKTSEIQSQ
AKAYFEKTHE QLTPLVRSAG TSLVNFFSSL MNLEEKPAPA
AK
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UCSC
uc007dnk.2; mouse.;
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