Mouse Apaf1 ELISA Kit

Alternative name
Mouse Apaf-1 ELISA Kit;Mouse 6230400I06Rik ELISA Kit;Mouse Apaf1l ELISA Kit;Mouse fog ELISA Kit;Mouse mKIAA0413 ELISA Kit;Mouse apoptotic peptidase activating factor 1 ELISA Kit;Mouse apoptotic protease-activating factor 1 ELISA Kit;Mouse forebrain overgrowth ELISA Kit;
Catalog
E044676
species
Mouse
GeneApaf1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 4.69pg/ml
Intended UseMouse Apaf1 ELISA Kit allows for the in vitro quantitative determination of Apaf1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse APAF1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse APAF1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse APAF1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse APAF1 can be calculated.
Mouse Apoptotic protease-activating factor 1 Protein information
Uniprot ID APAF_MOUSE
Uniprot AC O88879; A2RRK8;
UniGene Mm.220289;
GeneID 11783
KEGG mmu:11783;
Mouse Apoptotic protease-activating factor 1 Protein SEQUENCE
SEQUENCE 1249 AA; 141003 MW; AA639E407F9ABC24 CRC64;
MDAKARNCLL QHREALEKDI KTSYIMDHMI SNGVLSVIEE EKVKSQATQY
QRAAALIKMI LNKDNCAYIS FYNALLHEGY KDLAALLQSG LPLVSSSSGK
DTDGGITSFV RTVLCEGGVP QRPVIFVTRK KLVHAIQQKL WKLNGEPGWV
TIYGMAGCGK SVLAAEAVRD HSLLEGCFSG GVHWVSIGKQ DKSGLLMKLQ
NLCMRLDQEE SFSQRLPLNI EEAKDRLRVL MLRKHPRSLL ILDDVWDPWV
LKAFDNQCQI LLTTRDKSVT DSVMGPKHVV PVESGLGREK GLEILSLFVN
MKKEDLPAEA HSIIKECKGS PLVVSLIGAL LRDFPNRWAY YLRQLQNKQF
KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL
CVLWDLETEE VEDILQEFVN KSLLFCNRNG KSFCYYLHDL QVDFLTEKNR
SQLQDLHRKM VTQFQRYYQP HTLSPDQEDC MYWYNFLAYH MASANMHKEL
CALMFSLDWI KAKTELVGPA HLIHEFVAYR HILDEKDCAV CENFQEFLSL
NGHLLGRQPF PNIVQLGLCE PETSEVYRQA KLQAKQEGDT GRLYLEWINK
KTIKNLSRLV VRPHTDAVYH ACFSQDGQRI ASCGADKTLQ VFKAETGEKL
LDIKAHEDEV LCCAFSSDDS YIATCSADKK VKIWDSATGK LVHTYDEHSE
QVNCCHFTNK SNHLLLATGS NDFFLKLWDL NQKECRNTMF GHTNSVNHCR
FSPDDELLAS CSADGTLRLW DVRSANERKS INVKRFFLSS EDPPEDVEVI
VKCCSWSADG DKIIVAAKNK VLLFDIHTSG LLAEIHTGHH STIQYCDFSP
YDHLAVIALS QYCVELWNID SRLKVADCRG HLSWVHGVMF SPDGSSFLTA
SDDQTIRVWE TKKVCKNSAI VLKQEIDVVF QENETMVLAV DNIRGLQLIA
GKTGQIDYLP EAQVSCCCLS PHLEYVAFGD EDGAIKIIEL PNNRVFSSGV
GHKKAVRHIQ FTADGKTLIS SSEDSVIQVW NWQTGDYVFL QAHQETVKDF
RLLQDSRLLS WSFDGTVKVW NVITGRIERD FTCHQGTVLS CAISSDATKF
SSTSADKTAK IWSFDLLSPL HELKGHNGCV RCSAFSLDGI LLATGDDNGE
IRIWNVSDGQ LLHSCAPISV EEGTATHGGW VTDVCFSPDS KTLVSAGGYL
KWWNVATGDS SQTFYTNGTN LKKIHVSPDF RTYVTVDNLG ILYILQVLE
UCSC uc007gtg.1; mouse. [O88879-1];

Category

Mouse Apaf1 ELISA Kit

Mouse Apaf-1 ELISA Kit;Mouse 6230400I06Rik ELISA Kit;Mouse Apaf1l ELISA Kit;Mouse fog ELISA Kit;Mouse mKIAA0413 ELISA Kit;Mouse apoptotic peptidase activating factor 1 ELISA Kit;Mouse apoptotic protease-activating factor 1 ELISA Kit;Mouse forebrain overgrowth ELISA Kit;