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Mouse Atp4b ELISA Kit

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  • Alternative name

    Mouse gastric H(+)/K(+) ATPase subunit beta ELISA Kit;Mouse proton pump beta chain ELISA Kit;Mouse AV080843 ELISA Kit;Mouse ATPase, H+/K+ exchanging, beta polypeptide ELISA Kit;Mouse potassium-transporting ATPase subunit beta ELISA Kit;Mouse ATPase, H+/K+ transporting, beta polypeptide, gastric specific ELISA Kit;Mouse H+,K+-ATPase ELISA Kit;Mouse H+/K+-ATPase beta ELISA Kit;Mouse H,K-ATPase-Beta ELISA Kit;
  • Catalog
    E045247
  • species
    Mouse
  • GeneAtp4b
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATP4b. No significant cross-reactivity or interference between ATP4b and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse ATP4b is typically less than 0.066ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
  • Intended UseMouse Atp4b ELISA Kit allows for the in vitro quantitative determination of Atp4b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP4b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP4b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP4b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP4b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Potassium-transporting ATPase subunit beta Protein information
  • Uniprot ID ATP4B_MOUSE
  • Uniprot AC P50992; Q9D6S3; Q9D6T2; Q9D6Y2;
  • UniGene Mm.154039;
  • GeneID 11945
  • KEGG mmu:11945;
  • Mouse Potassium-transporting ATPase subunit beta Protein SEQUENCE

  • SEQUENCE 294 AA; 33566 MW; EAB8526C4C52431D CRC64;

    MAALQEKKSC SQRMAEFRHY CWNPDTGQML GRTPARWVWI SLYYAGFYVV

    MTGLFALCIY VLMQTIDPYT PDYQDQLKSP GVTLRPDVYG ERGLKISYNV

    SENSSWAGLT HTLHSFLAGY TPASQQDSIN CTSEKYFFQE SFAAPNHTKF

    SCKFTADMLQ NCSGLADPSF GFEEGKPCFI IKMNRIVKFL PSNNTAPRVD

    CTFQDDPQKP RKDTEPLQVE YYPPNGTFSL HYFPYYGKKA QPHYSNPLVA

    AKLLNVPKNM QVSIVCKILA DHVTFNNPHD PYEGKVEFKL TIQK

  • UCSC uc009kxu.1; mouse.;



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