Mouse Bag1 ELISA Kit

Alternative name
Mouse BAG-1 ELISA Kit;Mouse bcl-2-associated athanogene 1 ELISA Kit;Mouse Rap46 ELISA Kit;Mouse BCL2-associated athanogene 1 ELISA Kit;Mouse BAG family molecular chaperone regulator 1 ELISA Kit;Mouse Bcl-2-binding protein ELISA Kit;
Catalog
E045449
species
Mouse
GeneBag1
SpecificityThis assay has high sensitivity and excellent specificity for detection of BAG1. No significant cross-reactivity or interference between BAG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BAG1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Bag1 ELISA Kit allows for the in vitro quantitative determination of Bag1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: BAG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BAG1 antibody and an BAG1-HRP conjugate. The assay sample and buffer are incubated together with BAG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAG1 concentration since BAG1 from samples and BAG1-HRP conjugate compete for the anti-BAG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BAG1 from the sample, fewer sites are left to bind BAG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAG1 concentration in each sample is interpolated from this standard curve.
Mouse BAG family molecular chaperone regulator 1 Protein information
Uniprot ID BAG1_MOUSE
Uniprot AC Q60739; Q561N1; Q6IS45; Q9D7K6;
UniGene Mm.688;
GeneID 12017
KEGG mmu:12017;
Mouse BAG family molecular chaperone regulator 1 Protein SEQUENCE
SEQUENCE 355 AA; 39740 MW; 077A765CA869D3A7 CRC64;
MAGRSAARRP RGDREPLGPR LRAPRPAREP RQSESRAERG LPPSQRSSVR
SAASGHDRST RGAPAGACKP RVKKKVRPRS SQSEKVGSSS RELTRSKKVT
RSKNVTGTQV EEVTKIEEAT QTEEVTVAEE VTQTDNMAKT EEMVQTEEME
TPRLSVIVTH SNERYDLLVT PQQGNSEPVV QDLAQLVEEA TGVPLPFQKL
IFKGKSLKEM ETPLSALGMQ NGCRVMLIGE KSNPEEEVEL KKLKDLEVSA
EKIANHLQEL NKELSGIQQG FLAKELQAEA LCKLDRKVKA TIEQFMKILE
EIDTMVLPEQ FKDSRLKRKN LVKKVQVFLA ECDTVEQYIC QETERLQSTN
LALAE

Category

Mouse Bag1 ELISA Kit

Mouse BAG-1 ELISA Kit;Mouse bcl-2-associated athanogene 1 ELISA Kit;Mouse Rap46 ELISA Kit;Mouse BCL2-associated athanogene 1 ELISA Kit;Mouse BAG family molecular chaperone regulator 1 ELISA Kit;Mouse Bcl-2-binding protein ELISA Kit;