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Mouse Ca2 ELISA Kit

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  • Alternative name

    Mouse carbonate dehydratase II ELISA Kit;Mouse carbonic anhydrase II ELISA Kit;Mouse CA-II ELISA Kit;Mouse Car2 ELISA Kit;Mouse AI131712 ELISA Kit;Mouse CAII ELISA Kit;Mouse Ca2 ELISA Kit;Mouse Car-2 ELISA Kit;Mouse Ltw-5 ELISA Kit;Mouse Lvtw-5 ELISA Kit;Mouse carbonic anhydrase 2 ELISA Kit;
  • Catalog
    E046447
  • species
    Mouse
  • GeneCa2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CA2. No significant cross-reactivity or interference between Mouse CA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CA2 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.938ng/mL
  • Intended UseMouse Ca2 ELISA Kit allows for the in vitro quantitative determination of Ca2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CA2. You can calculate the concentration of Mouse CA2 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Carbonic anhydrase 2 Protein information
  • Uniprot ID CAH2_MOUSE
  • Uniprot AC P00920; Q6LDQ7; Q7TPE1; Q9DCT3; Q9DCY9;
  • UniGene Mm.1186;
  • GeneID 12349
  • KEGG mmu:12349;
  • Mouse Carbonic anhydrase 2 Protein SEQUENCE

  • SEQUENCE 260 AA; 29033 MW; 095760368D446AA6 CRC64;

    MSHHWGYSKH NGPENWHKDF PIANGDRQSP VDIDTATAQH DPALQPLLIS

    YDKAASKSIV NNGHSFNVEF DDSQDNAVLK GGPLSDSYRL IQFHFHWGSS

    DGQGSEHTVN KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKIG

    PASQGLQKVL EALHSIKTKG KRAAFANFDP CSLLPGNLDY WTYPGSLTTP

    PLLECVTWIV LREPITVSSE QMSHFRTLNF NEEGDAEEAM VDNWRPAQPL

    KNRKIKASFK

  • UCSC uc008oqt.2; mouse.;



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