Mouse Ca9 ELISA Kit

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  • Alternative name

    Mouse carbonate dehydratase IX ELISA Kit;Mouse carbonic anhydrase IX ELISA Kit;Mouse CA-IX ELISA Kit;Mouse CAIX ELISA Kit;Mouse membrane antigen MN homolog ELISA Kit;Mouse Car9 ELISA Kit;Mouse Ca9 ELISA Kit;Mouse MN/CA9 ELISA Kit;Mouse carbonic anhydrase 9 ELISA Kit;

  • Catalog
    E046452
  • species
    Mouse
  • GeneCa9
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CA9. No significant cross-reactivity or interference between Mouse CA9 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CA9 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity9.375pg/mL
  • Intended UseMouse Ca9 ELISA Kit allows for the in vitro quantitative determination of Ca9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA9 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CA9. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CA9. You can calculate the concentration of Mouse CA9 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Carbonic anhydrase 9 Protein information
  • Uniprot ID CAH9_MOUSE
  • Uniprot AC Q8VHB5; Q8K1G1; Q8VDE4;
  • UniGene Mm.283682;
  • GeneID 230099
  • KEGG mmu:230099;
  • Mouse Carbonic anhydrase 9 Protein SEQUENCE
  • SEQUENCE 437 AA; 47265 MW; 88F23380DCD35344 CRC64;

    MASLGPSPWA PLSTPAPTAQ LLLFLLLQVS AQPQGLSGMQ GEPSLGDSSS

    GEDELGVDVL PSEEDAPEEA DPPDGEDPPE VNSEDRMEES LGLEDLSTPE

    APEHSQGSHG DEKGGGHSHW SYGGTLLWPQ VSPACAGRFQ SPVDIRLERT

    AFCRTLQPLE LLGYELQPLP ELSLSNNGHT VQLTLPPGLK MALGPGQEYR

    ALQLHLHWGT SDHPGSEHTV NGHRFPAEIH VVHLSTAFSE LHEALGRPGG

    LAVLAAFLQE SPEENSAYEQ LLSHLEEISE EGSKIEIPGL DVSALLPSDL

    SRYYRYEGSL TTPPCSQGVI WTVFNETVKL SAKQLHTLSV SLWGPRDSRL

    QLNFRATQPL NGRTIEASFP AAEDSSPEPV HVNSCFTAGD ILALVFGLLF

    AVTSIAFLLQ LRRQHRHRSG TKDRVSYSPA EMTETGA

  • UCSC uc008sqa.1; mouse. [Q8VHB5-1];



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