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Mouse Camk2a ELISA Kit

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  • Alternative name

    Mouse caM kinase II subunit alpha ELISA Kit;Mouse caMK-II subunit alpha ELISA Kit;Mouse CaMKII ELISA Kit;Mouse R74975 ELISA Kit;Mouse mKIAA0968 ELISA Kit;Mouse calcium/calmodulin-dependent protein kinase II alpha ELISA Kit;Mouse calcium/calmodulin-dependent protein kinase type II subunit alpha ELISA Kit;Mouse CaMK II ELISA Kit;Mouse alpha-CaMKII ELISA Kit;
  • Catalog
    E046649
  • species
    Mouse
  • GeneCamk2a
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseMouse Camk2a ELISA Kit allows for the in vitro quantitative determination of Camk2a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha Protein information
  • Uniprot ID KCC2A_MOUSE
  • Uniprot AC P11798; Q61284; Q6ZWN4;
  • UniGene Mm.131530; Mm.446530;
  • GeneID 12322
  • KEGG mmu:12322;
  • Mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha Protein SEQUENCE

  • SEQUENCE 478 AA; 54115 MW; 306F416CCE9B5F62 CRC64;

    MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS

    ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED

    IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA

    AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG

    VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK

    MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK

    GAILTTMLAT RNFSGGKSGG NKKNDGVKES SESTNTTIED EDTKVRKQEI

    IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE

    NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE

    ETRVWHRRDG KWQIVHFHRS GAPSVLPH

  • UCSC uc008fbh.2; mouse. [P11798-2];



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