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Mouse Calr3 ELISA Kit

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  • Alternative name

    Mouse calreticulin-2 ELISA Kit;Mouse calsperin ELISA Kit;Mouse Crt2 ELISA Kit;Mouse 1700031L01Rik ELISA Kit;Mouse 6330586I20Rik ELISA Kit;Mouse cspn ELISA Kit;Mouse calreticulin 3 ELISA Kit;Mouse calreticulin-3 ELISA Kit;Mouse calspeculin ELISA Kit;
  • Catalog
    E046767
  • species
    Mouse
  • GeneCalr3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CALR3. No significant cross-reactivity or interference between CALR3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALR3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Calr3 ELISA Kit allows for the in vitro quantitative determination of Calr3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: CALR3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CALR3 antibody and an CALR3-HRP conjugate. The assay sample and buffer are incubated together with CALR3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALR3 concentration since CALR3 from samples and CALR3-HRP conjugate compete for the anti-CALR3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CALR3 from the sample, fewer sites are left to bind CALR3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALR3 concentration in each sample is interpolated from this standard curve.
  • Mouse Calreticulin-3 Protein information
  • Uniprot ID CALR3_MOUSE
  • Uniprot AC Q9D9Q6; G5E827;
  • UniGene Mm.196315;
  • GeneID 73316
  • KEGG mmu:73316;
  • Mouse Calreticulin-3 Protein SEQUENCE

  • SEQUENCE 380 AA; 44232 MW; B13366ADB11B0442 CRC64;

    MVSARALLWA ICVLRVALAT VYFQEEFLDG ERWRNRWVQS TNDSQFGHFR

    VSSGKFYGHK EKDKGLQTTQ NSRFYAISAS FKPFSNKGKT LVIQYTVKHE

    QKMDCGGGYI KVFPSDLDQK KMNGKSQYYI MFGPDICGFD IKKVHVILYF

    KNQYHENKKP IRCKVDGFTH LYTLILRPDL SYEVKVDGQS IESGSIEYDW

    NLTSLRKTEK TSLDSRDWDQ VEGSKVQDWE KHFLDAGASK PSDWNSELDG

    DWLQKPPYED GLKAEGIDKD VWLHQKMRPA GYLTQYDLSE FENIGAIGLE

    LWQVRSGTIF DNFLITDDEE YAEKFGKATW GETKGPEKEM DAIQAKEEVK

    KAREEDEEDL LMGKFHRHNH FSRFHRQGEL

  • UCSC uc009mfx.1; mouse.;



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