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Mouse Casp4 ELISA Kit

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  • Alternative name

    Mouse CASP-4 ELISA Kit;Mouse Caspase-11 ELISA Kit;Mouse CASP-11 ELISA Kit;Mouse protease ICH-3 ELISA Kit;Mouse Casp11 ELISA Kit;Mouse Caspl ELISA Kit;Mouse Ich3 ELISA Kit;Mouse ich-3 ELISA Kit;Mouse caspase 4, apoptosis-related cysteine peptidase ELISA Kit;Mouse caspase-4 ELISA Kit;Mouse caspase 11, apoptosis-related cysteine peptidase ELISA Kit;Mouse caspase 11, apoptosis-related cysteine protease ELISA Kit;Mouse caspase 4, apoptosis-related cysteine protease ELISA Kit;Mouse caspase-11 short form splicing ELISA Kit;
  • Catalog
    E047236
  • species
    Mouse
  • GeneCasp4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CASP4. No significant cross-reactivity or interference between Mouse CASP4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CASP4 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Casp4 ELISA Kit allows for the in vitro quantitative determination of Casp4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP4 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CASP4. You can calculate the concentration of Mouse CASP4 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Caspase-4 subunit p20 Protein information
  • Uniprot ID CASP4_MOUSE
  • Uniprot AC P70343; C6L648; O08735; Q3TAF3; Q6P8H1;
  • UniGene Mm.1569;
  • GeneID 12363
  • KEGG mmu:12363;
  • Mouse Caspase-4 subunit p20 Protein SEQUENCE

  • SEQUENCE 373 AA; 42742 MW; 110D7AA75E71C2B3 CRC64;

    MAENKHPDKP LKVLEQLGKE VLTEYLEKLV QSNVLKLKEE DKQKFNNAER

    SDKRWVFVDA MKKKHSKVGE MLLQTFFSVD PGSHHGEANL EMEEPEESLN

    TLKLCSPEEF TRLCREKTQE IYPIKEANGR TRKALIICNT EFKHLSLRYG

    ANFDIIGMKG LLEDLGYDVV VKEELTAEGM ESEMKDFAAL SEHQTSDSTF

    LVLMSHGTLH GICGTMHSEK TPDVLQYDTI YQIFNNCHCP GLRDKPKVII

    VQACRGGNSG EMWIRESSKP QLCRGVDLPR NMEADAVKLS HVEKDFIAFY

    STTPHHLSYR DKTGGSYFIT RLISCFRKHA CSCHLFDIFL KVQQSFEKAS

    IHSQMPTIDR ATLTRYFYLF PGN

  • UCSC uc009obt.3; mouse. [P70343-1];



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