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Mouse F3 ELISA Kit

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  • Alternative name

    Mouse TF ELISA Kit;Mouse coagulation factor III ELISA Kit;Mouse Cf-3 ELISA Kit;Mouse Cf3 ELISA Kit;Mouse AA409063 ELISA Kit;Mouse CD142 ELISA Kit;Mouse tissue factor ELISA Kit;
  • Catalog
    E047599
  • species
    Mouse
  • GeneF3
  • SpecificityThe Mouse CD142 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD142 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseMouse F3 ELISA Kit allows for the in vitro quantitative determination of F3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Mouse CD142 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD142 in Cell Culture Supernatants, Serum, Plasma, Cell Lysates. This assay employs an antibody specific for Mouse CD142 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD142 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD142 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD142 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
  • Mouse Tissue factor Protein information
  • Uniprot ID TF_MOUSE
  • Uniprot AC P20352;
  • UniGene Mm.273188;
  • GeneID 14066
  • KEGG mmu:14066;
  • Mouse Tissue factor Protein SEQUENCE

  • SEQUENCE 294 AA; 32935 MW; A306101293C31FA0 CRC64;

    MAILVRPRLL AALAPTFLGC LLLQVIAGAG IPEKAFNLTW ISTDFKTILE

    WQPKPTNYTY TVQISDRSRN WKNKCFSTTD TECDLTDEIV KDVTWAYEAK

    VLSVPRRNSV HGDGDQLVIH GEEPPFTNAP KFLPYRDTNL GQPVIQQFEQ

    DGRKLNVVVK DSLTLVRKNG TFLTLRQVFG KDLGYIITYR KGSSTGKKTN

    ITNTNEFSID VEEGVSYCFF VQAMIFSRKT NQNSPGSSTV CTEQWKSFLG

    ETLIIVGAVV LLATIFIILL SISLCKRRKN RAGQKGKNTP SRLA

  • UCSC uc008ree.2; mouse.;



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