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Alternative name
Low affinity immunoglobulin epsilon Fc receptor ELISA KIT; Fc-epsilon-RII ELISA KIT; Lymphocyte IgE receptor ELISA KIT; CD_antigen: CD23Fcer2 ELISA KIT; Fcer2a ELISA KIT
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Catalog
E047649
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species
Mouse
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GeneFcer2a
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SpecificityNatural and recombinant mouse CD23
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity Sample For quantitative detection of mouse CD23 in cell culture supernates, serum and plasm
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Intended UseMouse Fcer2a ELISA Kit allows for the in vitro quantitative determination of Fcer2a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: Mouse CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E50-P331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD23 amount of sample captured in plate.
Background: CD23, also known as Fc epsilon RII, or FcuRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
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