Mouse Nrp1 ELISA Kit

Alternative name
Mouse A5 protein ELISA Kit;Mouse Nrp ELISA Kit;Mouse C530029I03 ELISA Kit;Mouse NP-1 ELISA Kit;Mouse NPN-1 ELISA Kit;Mouse Npn1 ELISA Kit;Mouse neuropilin 1 ELISA Kit;Mouse neuropilin-1 ELISA Kit;Mouse Neuropilin-1 precursor (A5 protein) ELISA Kit;Mouse transmembrane receptor ELISA Kit;
Catalog
E047683
species
Mouse
GeneNrp1
SpecificityThe Mouse CD304 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD304 proteins within the range of 780 pg/ml - 50000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity20 pg/ml.
Intended UseMouse Nrp1 ELISA Kit allows for the in vitro quantitative determination of Nrp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Mouse CD304 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD304 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD304 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD304 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD304 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD304 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
Mouse Neuropilin-1 Protein information
Uniprot ID NRP1_MOUSE
Uniprot AC P97333; Q6PAR3;
UniGene Mm.271745;
GeneID 18186
KEGG mmu:18186;
Mouse Neuropilin-1 Protein SEQUENCE
SEQUENCE 923 AA; 103000 MW; 23209818C5C42137 CRC64;
MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP
SEKCEWLIQA PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW
GKFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN
YTAPTGVIKS PGFPEKYPNS LECTYIIFAP KMSEIILEFE SFDLEQDSNP
PGGMFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS GVLSMVFYTD
SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN
WSVERSRLNY PENGWTPGED SYKEWIQVDL GLLRFVTAVG TQGAISKETK
KKYYVKTYRV DISSNGEDWI SLKEGNKAII FQGNTNPTDV VLGVFSKPLI
TRFVRIKPVS WETGISMRFE VYGCKITDYP CSGMLGMVSG LISDSQITAS
NQADRNWMPE NIRLVTSRTG WALPPSPHPY TNEWLQVDLG DEKIVRGVII
QGGKHRENKV FMRKFKIAYS NNGSDWKTIM DDSKRKAKSF EGNNNYDTPE
LRTFSPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEAPTA GPTTPNGNPV
DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC
EFGWGSHKTF CHWEHDSHAQ LRWSVLTSKT GPIQDHTGDG NFIYSQADEN
QKGKVARLVS PVVYSQSSAH CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ
LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI FEGEIGKGNL GGIAVDDISI
NNHISQEDCA KPTDLDKKNT EIKIDETGST PGYEGEGEGD KNISRKPGNV
LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN
FELVDGVKLK KDKLNPQSNY SEA
UCSC uc009nzr.2; mouse.;

Category

Mouse Nrp1 ELISA Kit

Mouse A5 protein ELISA Kit;Mouse Nrp ELISA Kit;Mouse C530029I03 ELISA Kit;Mouse NP-1 ELISA Kit;Mouse NPN-1 ELISA Kit;Mouse Npn1 ELISA Kit;Mouse neuropilin 1 ELISA Kit;Mouse neuropilin-1 ELISA Kit;Mouse Neuropilin-1 precursor (A5 protein) ELISA Kit;Mouse transmembrane receptor ELISA Kit;