Mouse Cd4 ELISA Kit

Alternative name
Mouse T-cell differentiation antigen L3T4 ELISA Kit;Mouse T-cell surface antigen T4/Leu-3 ELISA Kit;Mouse L3T4 ELISA Kit;Mouse Ly-4 ELISA Kit;Mouse CD4 antigen ELISA Kit;Mouse T-cell surface glycoprotein CD4 ELISA Kit;Mouse T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4) ELISA Kit;
Catalog
E047704
species
Mouse
GeneCd4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CD4. No significant cross-reactivity or interference between Mouse CD4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CD4 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.234ng/mL
Intended UseMouse Cd4 ELISA Kit allows for the in vitro quantitative determination of Cd4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD4 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CD4. You can calculate the concentration of Mouse CD4 in the samples by comparing the OD of the samples to the standard curve.
Mouse T-cell surface glycoprotein CD4 Protein information
Uniprot ID CD4_MOUSE
Uniprot AC P06332;
UniGene Mm.2209;
GeneID 12504
KEGG mmu:12504;
Mouse T-cell surface glycoprotein CD4 Protein SEQUENCE
SEQUENCE 457 AA; 51297 MW; 1B1DA7527CB00F33 CRC64;
MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT
VFTWKFSDQR KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN
KLKMEDSQTY ICELENRKEE VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS
KVSNPLTECK HKKGKVVSGS KVLSMSNLRV QDSDFWNCTV TLDQKKNWFG
MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL MWKAEKDSFF
QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT
LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR
VSEEQKVVQV VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV
LGGSFGFLGF LGLCILCCVR CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM
QKSHNLI
UCSC uc012esr.1; mouse. [P06332-2];

Category

Mouse Cd4 ELISA Kit