Mouse Cd81 ELISA Kit

Alternative name
Mouse 26 kDa cell surface protein TAPA-1 ELISA Kit;Mouse target of the antiproliferative antibody 1 ELISA Kit;Mouse Tapa1 ELISA Kit;Mouse Tapa-1 ELISA Kit;Mouse Tspan28 ELISA Kit;Mouse CD81 antigen ELISA Kit;
Catalog
E047772
species
Mouse
GeneCd81
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD81. No significant cross-reactivity or interference between CD81 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD81 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cd81 ELISA Kit allows for the in vitro quantitative determination of Cd81 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This CD81 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD81. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CD81 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD81 antibody and an CD81-HRP conjugate. The assay sample and buffer are incubated together with CD81-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD81 concentration since CD81 from samples and CD81-HRP conjugate compete for the anti-CD81 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD81 from the sample, fewer sites are left to bind CD81-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD81 concentration in each sample is interpolated from this standard curve.
Mouse CD81 antigen Protein information
Uniprot ID CD81_MOUSE
Uniprot AC P35762; Q91V78;
UniGene Mm.806;
GeneID 12520
KEGG mmu:12520;
Mouse CD81 antigen Protein SEQUENCE
SEQUENCE 236 AA; 25815 MW; 7BDCB792E7F9FA4E CRC64;
MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTSLLYLELG
NKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI
LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVMDDDA NNAKAVVKTF
HETLNCCGSN ALTTLTTTIL RNSLCPSGGN ILTPLLQQDC HQKIDELFSG
KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY
UCSC uc009kou.2; mouse.;

Category

Mouse Cd81 ELISA Kit

Mouse 26 kDa cell surface protein TAPA-1 ELISA Kit;Mouse target of the antiproliferative antibody 1 ELISA Kit;Mouse Tapa1 ELISA Kit;Mouse Tapa-1 ELISA Kit;Mouse Tspan28 ELISA Kit;Mouse CD81 antigen ELISA Kit;