Mouse Cfb ELISA Kit

Alternative name
Mouse C3/C5 convertase ELISA Kit;Mouse Bf ELISA Kit;Mouse H2-Bf ELISA Kit;
Catalog
E048037
species
Mouse
GeneCfb
SpecificityThis kit recognizes natural and recombinant Mouse CFB. No significant cross-reactivity or interference between Mouse CFB and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.938ng/mL
Intended UseMouse Cfb ELISA Kit allows for the in vitro quantitative determination of Cfb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CFB concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CFB. You can calculate the concentration of CFB in the samples by comparing the OD of the samples to the standard curve.
Mouse Complement factor B Bb fragment Protein information
Uniprot ID CFAB_MOUSE
Uniprot AC P04186;
UniGene Mm.653;
Mouse Complement factor B Bb fragment Protein SEQUENCE
SEQUENCE 761 AA; 85004 MW; 9E9015448A61020E CRC64;
MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ
GGQALEYLCP SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC
PRPQDFENGE FWPRSPFYNL SDQISFQCYD GYVLRGSANR TCQENGRWDG
QTAICDDGAG YCPNPGIPIG TRKVGSQYRL EDIVTYHCSR GLVLRGSQKR
KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE GADAEDGHSP
GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY
GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT
NTKRALQAVY SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ
DIRALLDIGR DPKNPREDYL DVYVFGVGPL VDSVNINALA SKKDNEHHVF
KVKDMEDLEN VFYQMIDETK SLSLCGMVWE HKKGNDYHKQ PWQAKISVTR
PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG QRRDLEIEEV
LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT
TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK
ASCERDATKA QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL
IVHKRSRFIQ VGVISWGVVD VCRDQRRQQL VPSYARDFHI NLFQVLPWLK
DKLKDEDLGF L

Category

Mouse Cfb ELISA Kit

Mouse C3/C5 convertase ELISA Kit;Mouse Bf ELISA Kit;Mouse H2-Bf ELISA Kit;