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Mouse Pde5a ELISA Kit

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  • Alternative name

    Mouse cGMP-binding cGMP-specific phosphodiesterase ELISA Kit;Mouse CGB-PDE ELISA Kit;Mouse Pde5 ELISA Kit;Mouse Cgbpde ELISA Kit;Mouse Cn5n ELISA Kit;Mouse PDE5a2 ELISA Kit;Mouse Pde5a1 ELISA Kit;Mouse phosphodiesterase 5A, cGMP-specific ELISA Kit;Mouse cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit;
  • Catalog
    E048047
  • species
    Mouse
  • GenePde5a
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<46.9pg/ml
  • Intended UseMouse Pde5a ELISA Kit allows for the in vitro quantitative determination of Pde5a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Pde5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Pde5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Pde5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Pde5a can be calculated.
  • Mouse cGMP-specific 3',5'-cyclic phosphodiesterase Protein information
  • Uniprot ID PDE5A_MOUSE
  • Uniprot AC Q8CG03; Q0VBW0;
  • UniGene Mm.134911;
  • GeneID 242202
  • KEGG mmu:242202;
  • Mouse cGMP-specific 3',5'-cyclic phosphodiesterase Protein SEQUENCE

  • SEQUENCE 865 AA; 98407 MW; BADD21CC6B62045E CRC64;

    MERAGPNSVR SQQQRDPDWV EAWLDDHRDF TFSYFIRKAT RDMVNAWFSE

    RVHNIPVCKE GIRAHTESCS CSLQQSPHAD NTTPGAPARK ISASEFDRPL

    RPIVVKDSEG TVSFLSDSGK KEQMPLTPPR FDSDEGDQCS RLLELVKDIS

    SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSKDKFL ISRLFDVAEG

    STLEEASNNC IRLEWNKGIV GHVAAFGEPL NIKDAYEDPR FNAEVDQITG

    YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE KDFAAYLAFC

    GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI LKKIAATIIS

    FMQVQKCTIF IVDEDCPDSF SRVFHMECEE VGKPSDPLTR EQDANKINYM

    YAQYVKNTME PLNIPDVTKD KRFPWTNENM GHVNTPCIGS LLCTPIKNGK

    KNKVIGVCQL VNKMEENTGK IKAFNQNDEQ FLEAFVIFCG LGIQNTQMYE

    AVERAMAKQM VTLEVLSYHA SAAEEETREL QALSAAVVPS AQTLKITDFS

    FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL SVKKNYRKNV

    AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LETLALLIAA LSHDLDHRGV

    NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI LSGLSIDEYK

    TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFSFEDPL QKELFLAMLM

    TACDLSAITK PWPIQQRIAE LVAAEFFDQG DRERKELNME PADLMNREKK

    NKIPSMQVGF IDAICLQLYE ALTHVSEDCL PLLDGCRKNR QKWQALAEQQ

    EKMLLNGESS QGKRD

  • UCSC uc008ret.2; mouse.;



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