Mouse Cx3cr1 ELISA Kit

Alternative name
Mouse C-X3-C CKR-1 ELISA Kit;Mouse CX3CR1 ELISA Kit;Mouse fractalkine receptor ELISA Kit;Mouse chemokine (C-X3-C motif) receptor 1 ELISA Kit;Mouse CX3C chemokine receptor 1 ELISA Kit;Mouse chemokine (C-X3-C) receptor 1 ELISA Kit;
Catalog
E048189
species
Mouse
GeneCx3cr1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cx3cr1 ELISA Kit allows for the in vitro quantitative determination of Cx3cr1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: CX3CR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX3CR1 antibody and an CX3CR1-HRP conjugate. The assay sample and buffer are incubated together with CX3CR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX3CR1 concentration since CX3CR1 from samples and CX3CR1-HRP conjugate compete for the anti-CX3CR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX3CR1 from the sample, fewer sites are left to bind CX3CR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX3CR1 concentration in each sample is interpolated from this standard curve.
Mouse CX3C chemokine receptor 1 Protein information
Uniprot ID CX3C1_MOUSE
Uniprot AC Q9Z0D9;
UniGene Mm.44065;
GeneID 13051
KEGG mmu:13051;
Mouse CX3C chemokine receptor 1 Protein SEQUENCE
SEQUENCE 354 AA; 40266 MW; 12C745E8E3755CA9 CRC64;
MSTSFPELDL ENFEYDDSAE ACYLGDIVAF GTIFLSVFYA LVFTFGLVGN
LLVVLALTNS RKPKSITDIY LLNLALSDLL FVATLPFWTH YLISHEGLHN
AMCKLTTAFF FIGFFGGIFF ITVISIDRYL AIVLAANSMN NRTVQHGVTI
SLGVWAAAIL VASPQFMFTK RKDNECLGDY PEVLQEMWPV LRNSEVNILG
FALPLLIMSF CYFRIIQTLF SCKNRKKARA VRLILLVVFA FFLFWTPYNI
MIFLETLKFY NFFPSCDMKR DLRLALSVTE TVAFSHCCLN PFIYAFAGEK
FRRYLGHLYR KCLAVLCGHP VHTGFSPESQ RSRQDSILSS FTHYTSEGDG
SLLL
UCSC uc009sbz.2; mouse.;

Category

Mouse Cx3cr1 ELISA Kit

Mouse C-X3-C CKR-1 ELISA Kit;Mouse CX3CR1 ELISA Kit;Mouse fractalkine receptor ELISA Kit;Mouse chemokine (C-X3-C motif) receptor 1 ELISA Kit;Mouse CX3C chemokine receptor 1 ELISA Kit;Mouse chemokine (C-X3-C) receptor 1 ELISA Kit;