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Alternative name
UPF0556 protein C19orf10 ELISA KIT; Interleukin-25 ELISA KIT; IL-25 ELISA KIT; Stromal cell-derived growth factor SF20C19orf10 ELISA KIT; IL25 ELISA KIT; IL-25 ELISA KIT
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Catalog
E048475
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species
Mouse
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GeneC19orf10
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse IL25. No significant cross-reactivity or interference between mouse IL25 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity3.9 pg/ml.
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Intended UseMouse C19orf10 ELISA Kit allows for the in vitro quantitative determination of C19orf10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL25 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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