Mouse Ckm ELISA Kit

Alternative name
Mouse creatine kinase M chain ELISA Kit;Mouse M-CK ELISA Kit;Mouse Ckmm ELISA Kit;Mouse MCK ELISA Kit;Mouse creatine kinase, muscle ELISA Kit;Mouse creatine kinase M-type ELISA Kit;
Catalog
E048552
species
Mouse
GeneCkm
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CKM. No significant cross-reactivity or interference between Mouse CKM and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CKM and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.938ng/mL
Intended UseMouse Ckm ELISA Kit allows for the in vitro quantitative determination of Ckm , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CKM in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CKM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CKM. You can calculate the concentration of Mouse CKM in the samples by comparing the OD of the samples to the standard curve.
Mouse Creatine kinase M-type Protein information
Uniprot ID KCRM_MOUSE
Uniprot AC P07310;
UniGene Mm.2375;
GeneID 12715
KEGG mmu:12715;
Mouse Creatine kinase M-type Protein SEQUENCE
SEQUENCE 381 AA; 43045 MW; B573FEB1D2A41056 CRC64;
MPFGNTHNKF KLNYKPQEEY PDLSKHNNHM AKVLTPDLYN KLRDKETPSG
FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYTVFKDLFD PIIQDRHGGY
KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE
RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EQEQQQLIDD HFLFDKPVSP
LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF
RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLA
NLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GAVFDISNAD RLGSSEVEQV
QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K
UCSC uc009flu.1; mouse.;

Category

Mouse Ckm ELISA Kit

Mouse creatine kinase M chain ELISA Kit;Mouse M-CK ELISA Kit;Mouse Ckmm ELISA Kit;Mouse MCK ELISA Kit;Mouse creatine kinase, muscle ELISA Kit;Mouse creatine kinase M-type ELISA Kit;