Mouse Cldn18 ELISA Kit

Alternative name
Mouse claudin 18 ELISA Kit;Mouse claudin-18 ELISA Kit;
Catalog
E048610
species
Mouse
GeneCldn18
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLDN18. No significant cross-reactivity or interference between CLDN18 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN18 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cldn18 ELISA Kit allows for the in vitro quantitative determination of Cldn18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: CLDN18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLDN18 antibody and an CLDN18-HRP conjugate. The assay sample and buffer are incubated together with CLDN18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN18 concentration since CLDN18 from samples and CLDN18-HRP conjugate compete for the anti-CLDN18 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN18 from the sample, fewer sites are left to bind CLDN18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN18 concentration in each sample is interpolated from this standard curve.
Mouse Claudin-18 Protein information
Uniprot ID CLD18_MOUSE
Uniprot AC P56857; Q91ZY9; Q91ZZ0; Q91ZZ1;
UniGene Mm.386784;
GeneID 56492
KEGG mmu:56492;
Mouse Claudin-18 Protein SEQUENCE
SEQUENCE 264 AA; 28122 MW; 3CA0D441C4705653 CRC64;
MATTTCQVVG LLLSLLGLAG CIAATGMDMW STQDLYDNPV TAVFQYEGLW
RSCVQQSSGF TECRPYFTIL GLPAMLQAVR ALMIVGIVLG VIGILVSIFA
LKCIRIGSMD DSAKAKMTLT SGILFIISGI CAIIGVSVFA NMLVTNFWMS
TANMYSGMGG MGGMVQTVQT RYTFGAALFV GWVAGGLTLI GGVMMCIACR
GLTPDDSNFK AVSYHASGQN VAYRPGGFKA STGFGSNTRN KKIYDGGART
EDDEQSHPTK YDYV
UCSC uc009res.2; mouse. [P56857-3];

Category

Mouse Cldn18 ELISA Kit

Mouse claudin 18 ELISA Kit;Mouse claudin-18 ELISA Kit;