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Mouse Cd300a ELISA Kit

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  • Alternative name

    Mouse CLM-8 ELISA Kit;Mouse CD300 antigen-like family member A ELISA Kit;Mouse leukocyte mono-Ig-like receptor 1 ELISA Kit;Mouse mast cell-derived paired immunoglobulin-like receptor 1 ELISA Kit;Mouse myeloid-associated immunoglobulin-like receptor 1 ELISA Kit;Mouse MAIR-1 ELISA Kit;Mouse MAIR-I ELISA Kit;Mouse Clm8 ELISA Kit;Mouse LMIR1 ELISA Kit;Mouse mcpir1 ELISA Kit;Mouse B230315M08Rik ELISA Kit;Mouse MAIR-Ia ELISA Kit;Mouse MMAC8 ELISA Kit;Mouse Pigr4 ELISA Kit;Mouse CD300A molecule ELISA Kit;Mouse CMRF35-like molecule 8 ELISA Kit;Mouse CD300A antigen ELISA Kit;Mouse polymeric immunoglobulin receptor 4 ELISA Kit;
  • Catalog
    E048741
  • species
    Mouse
  • GeneCd300a
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD300A. No significant cross-reactivity or interference between CD300A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD300A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Cd300a ELISA Kit allows for the in vitro quantitative determination of Cd300a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the assay: CD300A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD300A antibody and an CD300A-HRP conjugate. The assay sample and buffer are incubated together with CD300A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD300A concentration since CD300A from samples and CD300A-HRP conjugate compete for the anti-CD300A antibody binding site. Since the number of sites is limited, as more sites are occupied by CD300A from the sample, fewer sites are left to bind CD300A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD300A concentration in each sample is interpolated from this standard curve.
  • Mouse CMRF35-like molecule 8 Protein information
  • Uniprot ID CLM8_MOUSE
  • Uniprot AC Q6SJQ0; B9EIB1; Q7TN56; Q7TSN3; Q8CFN3;
  • UniGene Mm.294439;
  • GeneID 217303
  • KEGG mmu:217303;
  • Mouse CMRF35-like molecule 8 Protein SEQUENCE

  • SEQUENCE 318 AA; 35629 MW; 490EDBD46AF0A7C6 CRC64;

    MTQLASAVWL PTLLLLLLLF WLPGCVPLHG PSTMSGSVGE SLSVSCRYEE

    KFKTKDKYWC RVSLKILCKD IVKTSSSEEA RSGRVTIRDH PDNLTFTVTY

    ESLTLEDADT YMCAVDISLF DGSLGFDKYF KIELSVVPSE DPVSSPGPTL

    ETPVVSTSLP TKGPALGSNT EGHREHDYSQ GLRLPALLSV LALLLFLLVG

    TSLLAWRMFQ KRLVKADRHP ELSQNLRQAS EQNECQYVNL QLHTWSLREE

    PVLPSQVEVV EYSTLALPQE ELHYSSVAFN SQRQDSHANG DSLHQPQDQK

    AEYSEIQKPR KGLSDLYL

  • UCSC uc007mfz.1; mouse. [Q6SJQ0-2];



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