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Mouse Commd5 ELISA Kit

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  • Alternative name

    Mouse 2310065H03Rik ELISA Kit;Mouse AI854466 ELISA Kit;Mouse D15Ertd81e ELISA Kit;Mouse Hcarg ELISA Kit;Mouse COMM domain containing 5 ELISA Kit;Mouse COMM domain-containing protein 5 ELISA Kit;Mouse hypertension-related, calcium regulated ELISA Kit;
  • Catalog
    E049259
  • species
    Mouse
  • GeneCommd5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of COMMD5. No significant cross-reactivity or interference between COMMD5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COMMD5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Commd5 ELISA Kit allows for the in vitro quantitative determination of Commd5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This COMMD5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse COMMD5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||COMMD5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COMMD5 antibody and an COMMD5-HRP conjugate. The assay sample and buffer are incubated together with COMMD5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COMMD5 concentration since COMMD5 from samples and COMMD5-HRP conjugate compete for the anti-COMMD5 antibody binding site. Since the number of sites is limited, as more sites are occupied by COMMD5 from the sample, fewer sites are left to bind COMMD5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COMMD5 concentration in each sample is interpolated from this standard curve.
  • Mouse COMM domain-containing protein 5 Protein information
  • Uniprot ID COMD5_MOUSE
  • Uniprot AC Q8R395; Q9D6R8;
  • UniGene Mm.291716;
  • GeneID 66398
  • KEGG mmu:66398;
  • Mouse COMM domain-containing protein 5 Protein SEQUENCE

  • SEQUENCE 224 AA; 24493 MW; 423091DFE266DE18 CRC64;

    MSALGAPAPY LHHPTDSHSG RVSFLGSQPS AEVTAVAQLL KDLDRSTFRK

    LLKLVVGALH GKDCREAVQH LGASANLSEE RLAVLLAGTH TLLQQALRLP

    PASLKPDAFQ DELQELGIPQ DMIGDLASLA FGSQRPLLDS VAQQQGSSLP

    RVSNFRWRVD VAISTSAQSR SLQPSVLMQL KLTDGSAHRF EVPIAKFQEL

    RYSVALVLKE MAELERKCER KLQD

  • UCSC uc007wms.1; mouse.;



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