Mouse Ctgf ELISA Kit

Alternative name
Mouse CCN family member 2 ELISA Kit;Mouse hypertrophic chondrocyte-specific protein 24 ELISA Kit;Mouse Protein FISP-12 ELISA Kit;Mouse Ccn2 ELISA Kit;Mouse fisp-12 ELISA Kit;Mouse Fisp12 ELISA Kit;Mouse Hcs24 ELISA Kit;Mouse connective tissue growth factor ELISA Kit;Mouse Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24) ELISA Kit;Mouse fibroblast inducible secreated protein ELISA Kit;Mouse fibroblast inducible secreted protein ELISA Kit;Mouse hypertrophic chondrocyte-specific gene product 24 ELISA Kit;
Catalog
E049480
species
Mouse
GeneCtgf
SpecificityThis assay has high sensitivity and excellent specificity for detection of CTGF. No significant cross-reactivity or interference between CTGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTGF and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Ctgf ELISA Kit allows for the in vitro quantitative determination of Ctgf , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: CTGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTGF antibody and an CTGF-HRP conjugate. The assay sample and buffer are incubated together with CTGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTGF concentration since CTGF from samples and CTGF-HRP conjugate compete for the anti-CTGF antibody binding site. Since the number of sites is limited, as more sites are occupied by CTGF from the sample, fewer sites are left to bind CTGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTGF concentration in each sample is interpolated from this standard curve.
Mouse Connective tissue growth factor Protein information
Uniprot ID CTGF_MOUSE
Uniprot AC P29268; G5E830; Q922U0;
UniGene Mm.390287;
GeneID 14219
KEGG mmu:14219;
Mouse Connective tissue growth factor Protein SEQUENCE
SEQUENCE 348 AA; 37824 MW; 9BD99E6D0BEFC7BC CRC64;
MLASVAGPIS LALVLLALCT RPAMGQDCSA QCQCAAEAAP HCPAGVSLVL
DGCGCCRVCA KQLGELCTER DPCDPHKGLF CDFGSPANRK IGVCTAKDGA
PCVFGGSVYR SGESFQSSCK YQCTCLDGAV GCVPLCSMDV RLPSPDCPFP
RRVKLPGKCC EEWVCDEPKD RTAVGPALAA YRLEDTFGPD PTMMRANCLV
QTTEWSACSK TCGMGISTRV TNDNTFCRLE KQSRLCMVRP CEADLEENIK
KGKKCIRTPK IAKPVKFELS GCTSVKTYRA KFCGVCTDGR CCTPHRTTTL
PVEFKCPDGE IMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
UCSC uc011xbr.1; mouse.;

Category

Mouse Ctgf ELISA Kit