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Mouse Hsd11b2 ELISA Kit

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  • Alternative name

    Mouse 11-beta-hydroxysteroid dehydrogenase type 2 ELISA Kit;Mouse 11-DH2 ELISA Kit;Mouse 11-beta-HSD2 ELISA Kit;Mouse NAD-dependent 11-beta-hydroxysteroid dehydrogenase ELISA Kit;Mouse Hsd11k ELISA Kit;Mouse 11HSD2 ELISA Kit;Mouse hydroxysteroid 11-beta dehydrogenase 2 ELISA Kit;Mouse corticosteroid 11-beta-dehydrogenase isozyme 2 ELISA Kit;
  • Catalog
    E049534
  • species
    Mouse
  • GeneHsd11b2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HSD11B2. No significant cross-reactivity or interference between HSD11B2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSD11B2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Hsd11b2 ELISA Kit allows for the in vitro quantitative determination of Hsd11b2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: HSD11B2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSD11B2 antibody and an HSD11B2-HRP conjugate. The assay sample and buffer are incubated together with HSD11B2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSD11B2 concentration since HSD11B2 from samples and HSD11B2-HRP conjugate compete for the anti-HSD11B2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSD11B2 from the sample, fewer sites are left to bind HSD11B2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSD11B2 concentration in each sample is interpolated from this standard curve.
  • Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 Protein information
  • Uniprot ID DHI2_MOUSE
  • Uniprot AC P51661; Q91WK3;
  • UniGene Mm.5079;
  • GeneID 15484
  • KEGG mmu:15484;
  • Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 Protein SEQUENCE

  • SEQUENCE 386 AA; 42187 MW; 687FD253568487AB CRC64;

    MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR

    LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK

    KLDAMGFTVL ATVLDLNSPG ALELRDLCSP RLKLLQMDLT KAEDISRVLE

    ITKAHTASTG LWGLVNNAGL NIVVADVELS PVATFRKCME VNFFGALELT

    KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFGCEL

    LPWGIKVSII KPGCFKTDAV TNVNLWEKRK QLLLANIPRE LLQAYGEDYI

    EHVHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYP GRGLGLMYFI

    HHYLPEGLRR CFLQNFFINH LLPRALRPGQ HGPAPA

  • UCSC uc009nde.1; mouse.;



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