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Mouse Cryba1 ELISA Kit

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  • Alternative name

    Mouse Beta-A1-crystallin ELISA Kit;
  • Catalog
    E049723
  • species
    Mouse
  • GeneCryba1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Crystallin Beta A1 (CRYbA1). No significant cross-reactivity or interference between Crystallin Beta A1 (CRYbA1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseMouse Cryba1 ELISA Kit allows for the in vitro quantitative determination of Cryba1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Beta A1 (CRYbA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Beta A1 (CRYbA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Beta A1 (CRYbA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crystallin Beta A1 (CRYbA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Beta-crystallin A1 Protein information
  • Uniprot ID CRBA1_MOUSE
  • Uniprot AC P02525;
  • UniGene Mm.22830;
  • Mouse Beta-crystallin A1 Protein SEQUENCE

  • SEQUENCE 198 AA; 23401 MW; F7EBDD750402FEC5 CRC64;

    MLYLVLFLVP FNSIQITIYD QENFQGKRME FTSSCPNVSE RNFDNVRSLK

    VECGAWIGYE HTSFCGQQFI LERGEYPRWD AWSGSNAYHI ERLMSFRPIC

    SANHKESKIT IFEKENFIGR QWEICDDYPS LQAMGWFNNE VGSMKIQCGA

    WVCYQYPGYR GYQYILECDH HGGDYKHWPE WGSHAQTSQI QSIRRIQQ




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