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Alternative name
Mouse Cystatin-A1 ELISA Kit;Mouse stefin-A ELISA Kit;Mouse Csta1 ELISA Kit;Mouse Csta ELISA Kit;Mouse cystatin A1 ELISA Kit;Mouse cystatin-A ELISA Kit;
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Catalog
E049742
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species
Mouse
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GeneCsta
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CSTA. No significant cross-reactivity or interference between Mouse CSTA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CSTA and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity37.5pg/mL
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Intended UseMouse Csta ELISA Kit allows for the in vitro quantitative determination of Csta , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CSTA in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CSTA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CSTA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CSTA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CSTA. You can calculate the concentration of Mouse CSTA in the samples by comparing the OD of the samples to the standard curve.
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Mouse Cystatin-A, N-terminally processed Protein information
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Uniprot ID
CYTA_MOUSE
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Uniprot AC
P56567; Q3UV05;
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UniGene
Mm.300592;
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GeneID
209294
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KEGG
mmu:209294;
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Mouse Cystatin-A, N-terminally processed Protein SEQUENCE
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SEQUENCE 97 AA; 10929 MW; 5EDBAA66E81F76EC CRC64;
MIPGGLTEAR PATAEVQEIA DRVKAQLEEE TNEKYEIFKA VEYKTQVVAG
VNYFIKMDVG
GGCFTHIKVF KDLSGKNNLE LTGYQTNKTE DDELTYF
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UCSC
uc007zcf.1; mouse.;
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