Mouse Cyp1a2 ELISA Kit

Alternative name
Mouse CYPIA2 ELISA Kit;Mouse cholesterol 25-hydroxylase ELISA Kit;Mouse cytochrome P450-P2 ELISA Kit;Mouse cytochrome P450-P3 ELISA Kit;Mouse Cyp1a-2 ELISA Kit;Mouse CP12 ELISA Kit;Mouse Cyp1a1 ELISA Kit;Mouse P450-3 ELISA Kit;Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 ELISA Kit;Mouse cytochrome P450 1A2 ELISA Kit;Mouse aromatic compound inducible ELISA Kit;Mouse cytochrome P450 family 1 subfamily a polypeptide 1 ELISA Kit;Mouse cytochrome P450, 1a2, aromatic compound inducible ELISA Kit;
Catalog
E050056
species
Mouse
GeneCyp1a2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CYP1A2. No significant cross-reactivity or interference between Mouse CYP1A2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CYP1A2 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Cyp1a2 ELISA Kit allows for the in vitro quantitative determination of Cyp1a2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYP1A2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP1A2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CYP1A2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYP1A2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CYP1A2. You can calculate the concentration of Mouse CYP1A2 in the samples by comparing the OD of the samples to the standard curve.
Mouse Cytochrome P450 1A2 Protein information
Uniprot ID CP1A2_MOUSE
Uniprot AC P00186; Q9QWJ4;
UniGene Mm.15537;
GeneID 13077
KEGG mmu:13077;
Mouse Cytochrome P450 1A2 Protein SEQUENCE
SEQUENCE 513 AA; 58184 MW; DC2D7D976B126E3B CRC64;
MAFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI
GHMLTVGKNP HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS
DPTSASSCYL EEHVSKEANH LVSKLQKAMA EVGHFEPVSQ VVESVANVIG
AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV LRYLPNPALK
RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI
PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP
KERCIYINQW QVNHDEKQWK DPFVFRPERF LTNNNSAIDK TQSEKVMLFG
LGKRRCIGEI PAKWEVFLFL AILLQHLEFS VPPGVKVDLT PNYGLTMKPG
TCEHVQAWPR FSK
UCSC uc009pvm.1; mouse.;

Category

Mouse Cyp1a2 ELISA Kit