Mouse Mtco1 ELISA Kit

Alternative name
Mouse Cytochrome c oxidase polypeptide I ELISA Kit;Mouse COI ELISA Kit;Mouse mt-Co1 ELISA Kit;Mouse CoxI ELISA Kit;Mouse cytochrome c oxidase subunit I ELISA Kit;
Catalog
E050240
species
Mouse
GeneMtco1
SpecificityThis assay recognizes recombinant and natural mouse Cytochrome c oxidase subunit 1. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Mtco1 ELISA Kit allows for the in vitro quantitative determination of Mtco1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome c oxidase subunit 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome c oxidase subunit 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome c oxidase subunit 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cytochrome c oxidase subunit 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Cytochrome c oxidase subunit 1 Protein information
Uniprot ID COX1_MOUSE
Uniprot AC P00397;
GeneID 17708
KEGG mmu:17708;
Mouse Cytochrome c oxidase subunit 1 Protein SEQUENCE
SEQUENCE 514 AA; 56910 MW; 9F7FB173ED6A9763 CRC64;
MFINRWLFST NHKDIGTLYL LFGAWAGMVG TALSILIRAE LGQPGALLGD
DQIYNVIVTA HAFVMIFFMV MPMMIGGFGN WLVPLMIGAP DMAFPRMNNM
SFWLLPPSFL LLLASSMVEA GAGTGWTVYP PLAGNLAHAG ASVDLTIFSL
HLAGVSSILG AINFITTIIN MKPPAMTQYQ TPLFVWSVLI TAVLLLLSLP
VLAAGITMLL TDRNLNTTFF DPAGGGDPIL YQHLFWFFGH PEVYILILPG
FGIISHVVTY YSGKKEPFGY MGMVWAMMSI GFLGFIVWAH HMFTVGLDVD
TRAYFTSATM IIAIPTGVKV FSWLATLHGG NIKWSPAMLW ALGFIFLFTV
GGLTGIVLSN SSLDIVLHDT YYVVAHFHYV LSMGAVFAIM AGFVHWFPLF
SGFTLDDTWA KAHFAIMFVG VNMTFFPQHF LGLSGMPRRY SDYPDAYTTW
NTVSSMGSFI SLTAVLIMIF MIWEAFASKR EVMSVSYAST NLEWLHGCPP
PYHTFEEPTY VKVK

Category

Mouse Mtco1 ELISA Kit

Mouse Cytochrome c oxidase polypeptide I ELISA Kit;Mouse COI ELISA Kit;Mouse mt-Co1 ELISA Kit;Mouse CoxI ELISA Kit;Mouse cytochrome c oxidase subunit I ELISA Kit;