Mouse Des ELISA Kit

Alternative name
Mouse desmin ELISA Kit;
Catalog
E050817
species
Mouse
GeneDes
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse Des. No significant cross-reactivity or interference between Mouse Des and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse Des and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Des ELISA Kit allows for the in vitro quantitative determination of Des , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Des in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Des. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse Des and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Des, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Des. You can calculate the concentration of Mouse Des in the samples by comparing the OD of the samples to the standard curve.
Mouse Desmin Protein information
Uniprot ID DESM_MOUSE
Uniprot AC P31001;
UniGene Mm.6712;
GeneID 13346
KEGG mmu:13346;
Mouse Desmin Protein SEQUENCE
SEQUENCE 469 AA; 53498 MW; 58A8A13C7CC11EEF CRC64;
MSQAYSSSQR VSSYRRTFGG APGFSLGSPL SSPVFPRAGF GTKGSSSSMT
SRVYQVSRTS GGAGGLGSLR SSRLGTTRAP SYGAGELLDF SLADAVNQEF
LATRTNEKVE LQELNDRFAN YIEKVRFLEQ QNAALAAEVN RLKGREPTRV
AELYEEEMRE LRRQVEVLTN QRARVDVERD NLIDDLQRLK AKLQEEIQLR
EEAENNLAAF RADVDAATLA RIDLERRIES LNEEIAFLKK VHEEEIRELQ
AQLQEQQVQV EMDMSKPDLT AALRDIRAQY ETIAAKNISE AEEWYKSKVS
DLTQAANKNN DALRQAKQEM MEYRHQIQSY TCEIDALKGT NDSLMRQMRE
LEDRFASEAN GYQDNIARLE EEIRHLKDEM ARHLREYQDL LNVKMALDVE
IATYRKLLEG EESRINLPIQ TFSALNFRET SPEQRGSEVH TKKTVMIKTI
ETRDGEVVSE ATQQQHEVL
UCSC uc007box.2; mouse.;

Category

Mouse Des ELISA Kit

Mouse desmin ELISA Kit;