Mouse Dgat1 ELISA Kit

Alternative name
Mouse acyl-CoA retinol O-fatty-acyltransferase ELISA Kit;Mouse ARAT ELISA Kit;Mouse Retinol O-fatty-acyltransferase ELISA Kit;Mouse diglyceride acyltransferase ELISA Kit;Mouse Dgat ELISA Kit;Mouse C75990 ELISA Kit;Mouse D15Ertd23e ELISA Kit;Mouse diacylglycerol O-acyltransferase 1 ELISA Kit;
Catalog
E050868
species
Mouse
GeneDgat1
SpecificityThis assay has high sensitivity and excellent specificity for detection ofDGAT1 . No significant cross-reactivity or interference betweenDGAT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<11.2pg/ml
Intended UseMouse Dgat1 ELISA Kit allows for the in vitro quantitative determination of Dgat1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DGAT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DGAT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DGAT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofDGAT1 can be calculated.
Mouse Diacylglycerol O-acyltransferase 1 Protein information
Uniprot ID DGAT1_MOUSE
Uniprot AC Q9Z2A7; Q9D7Q5;
UniGene Mm.22633;
GeneID 13350
KEGG mmu:13350;
Mouse Diacylglycerol O-acyltransferase 1 Protein SEQUENCE
SEQUENCE 498 AA; 56790 MW; E7B0DD6DDCF1EC2B CRC64;
MGDRGGAGSS RRRRTGSRVS VQGGSGPKVE EDEVRDAAVS PDLGAGGDAP
APAPAPAHTR DKDGRTSVGD GYWDLRCHRL QDSLFSSDSG FSNYRGILNW
CVVMLILSNA RLFLENLIKY GILVDPIQVV SLFLKDPYSW PAPCVIIASN
IFVVAAFQIE KRLAVGALTE QMGLLLHVVN LATIICFPAA VALLVESITP
VGSVFALASY SIMFLKLYSY RDVNLWCRQR RVKAKAVSTG KKVSGAAAQQ
AVSYPDNLTY RDLYYFIFAP TLCYELNFPR SPRIRKRFLL RRVLEMLFFT
QLQVGLIQQW MVPTIQNSMK PFKDMDYSRI IERLLKLAVP NHLIWLIFFY
WFFHSCLNAV AELLQFGDRE FYRDWWNAES VTYFWQNWNI PVHKWCIRHF
YKPMLRHGSS KWVARTGVFL TSAFFHEYLV SVPLRMFRLW AFTAMMAQVP
LAWIVGRFFQ GNYGNAAVWV TLIIGQPVAV LMYVHDYYVL NYDAPVGV
UCSC uc007wkn.1; mouse.;

Category

Mouse Dgat1 ELISA Kit

Mouse acyl-CoA retinol O-fatty-acyltransferase ELISA Kit;Mouse ARAT ELISA Kit;Mouse Retinol O-fatty-acyltransferase ELISA Kit;Mouse diglyceride acyltransferase ELISA Kit;Mouse Dgat ELISA Kit;Mouse C75990 ELISA Kit;Mouse D15Ertd23e ELISA Kit;Mouse diacylglycerol O-acyltransferase 1 ELISA Kit;