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Mouse Dmd ELISA Kit

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  • Alternative name

    Mouse DXSmh7 ELISA Kit;Mouse DXSmh9 ELISA Kit;Mouse Dp427 ELISA Kit;Mouse Dp71 ELISA Kit;Mouse dys ELISA Kit;Mouse mdx ELISA Kit;Mouse pke ELISA Kit;Mouse dystrophin, muscular dystrophy ELISA Kit;Mouse dystrophin ELISA Kit;Mouse X-linked muscular dystrophy ELISA Kit;
  • Catalog
    E051083
  • species
    Mouse
  • GeneDmd
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse DMD. No significant cross-reactivity or interference between Mouse DMD and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse DMD and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseMouse Dmd ELISA Kit allows for the in vitro quantitative determination of Dmd , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DMD in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DMD. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse DMD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DMD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse DMD. You can calculate the concentration of Mouse DMD in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Dystrophin Protein information
  • Uniprot ID DMD_MOUSE
  • Uniprot AC P11531; A2A9Z0; O35653; Q60703;
  • UniGene Mm.275608; Mm.416750;
  • GeneID 13405
  • KEGG mmu:13405;
  • Mouse Dystrophin Protein SEQUENCE

  • SEQUENCE 3678 AA; 425832 MW; 53487B1E27104228 CRC64;

    MLWWEEVEDC YEREDVQKKT FTKWINAQFS KFGKQHIDNL FSDLQDGKRL

    LDLLEGLTGQ KLPKEKGSTR VHALNNVNKA LRVLQKNNVD LVNIGSTDIV

    DGNHKLTLGL IWNIILHWQV KNVMKTIMAG LQQTNSEKIL LSWVRQSTRN

    YPQVNVINFT SSWSDGLALN ALIHSHRPDL FDWNSVVSQH SATQRLEHAF

    NIAKCQLGIE KLLDPEDVAT TYPDKKSILM YITSLFQVLP QQVSIEAIQE

    VEMLPRTSSK VTREEHFQLH HQMHYSQQIT VSLAQGYEQT SSSPKPRFKS

    YAFTQAAYVA TSDSTQSPYP SQHLEAPRDK SLDSSLMETE VNLDSYQTAL

    EEVLSWLLSA EDTLRAQGEI SNDVEEVKEQ FHAHEGFMMD LTSHQGLVGN

    VLQLGSQLVG KGKLSEDEEA EVQEQMNLLN SRWECLRVAS MEKQSKLHKV

    LMDLQNQKLK ELDDWLTKTE ERTKKMEEEP FGPDLEDLKC QVQQHKVLQE

    DLEQEQVRVN SLTHMVVVVD ESSGDHATAA LEEQLKVLGD RWANICRWTE

    DRWIVLQDIL LKWQHFTEEQ CLFSTWLSEK EDAMKNIQTS GFKDQNEMMS

    SLHKISTLKI DLEKKKPTME KLSSLNQDLL SALKNKSVTQ KMEIWMENFA

    QRWDNLTQKL EKSSAQISQA VTTTQPSLTQ TTVMETVTMV TTREQIMVKH

    AQEELPPPPP QKKRQITVDS ELRKRLDVDI TELHSWITRS EAVLQSSEFA

    VYRKEGNISD LQEKVNAIAR EKAEKFRKLQ DASRSAQALV EQMANEGVNA

    ESIRQASEQL NSRWTEFCQL LSERVNWLEY QTNIITFYNQ LQQLEQMTTT

    AENLLKTQST TLSEPTAIKS QLKICKDEVN RLSALQPQIE QLKIQSLQLK

    EKGQGPMFLD ADFVAFTNHF NHIFDGVRAK EKELQTIFDT LPPMRYQETM

    SSIRTWIQQS ESKLSVPYLS VTEYEIMEER LGKLQALQSS LKEQQNGFNY

    LSDTVKEMAK KAPSEICQKY LSEFEEIEGH WKKLSSQLVE SCQKLEEHMN

    KLRKFQNHIK TLQKWMAEVD VFLKEEWPAL GDAEILKKQL KQCRLLVGDI

    QTIQPSLNSV NEGGQKIKSE AELEFASRLE TELRELNTQW DHICRQVYTR

    KEALKAGLDK TVSLQKDLSE MHEWMTQAEE EYLERDFEYK TPDELQTAVE

    EMKRAKEEAL QKETKVKLLT ETVNSVIAHA PPSAQEALKK ELETLTTNYQ

    WLCTRLNGKC KTLEEVWACW HELLSYLEKA NKWLNEVELK LKTMENVPAG

    PEEITEVLES LENLMHHSEE NPNQIRLLAQ TLTDGGVMDE LINEELETFN

    SRWRELHEEA VRKQKLLEQS IQSAQEIEKS LHLIQESLEF IDKQLAAYIT

    DKVDAAQMPQ EAQKIQSDLT SHEISLEEMK KHNQGKDANQ RVLSQIDVAQ

    KKLQDVSMKF RLFQKPANFE QRLEESKMIL DEVKMHLPAL ETKSVEQEVI

    QSQLSHCVNL YKSLSEVKSE VEMVIKTGRQ IVQKKQTENP KELDERVTAL

    KLHYNELGAK VTERKQQLEK CLKLSRKMRK EMNVLTEWLA ATDTELTKRS

    AVEGMPSNLD SEVAWGKATQ KEIEKQKAHL KSVTELGESL KMVLGKKETL

    VEDKLSLLNS NWIAVTSRVE EWLNLLLEYQ KHMETFDQNI EQITKWIIHA

    DELLDESEKK KPQQKEDILK RLKAEMNDMR PKVDSTRDQA AKLMANRGDH

    CRKVVEPQIS ELNRRFAAIS HRIKTGKASI PLKELEQFNS DIQKLLEPLE

    AEIQQGVNLK EEDFNKDMSE DNEGTVNELL QRGDNLQQRI TDERKREEIK

    IKQQLLQTKH NALKDLRSQR RKKALEISHQ WYQYKRQADD LLKCLDEIEK

    KLASLPEPRD ERKLKEIDRE LQKKKEELNA VRRQAEGLSE NGAAMAVEPT

    QIQLSKRWRQ IESNFAQFRR LNFAQIHTLH EETMVVTTED MPLDVSYVPS

    TYLTEISHIL QALSEVDHLL NTPELCAKDF EDLFKQEESL KNIKDNLQQI

    SGRIDIIHKK KTAALQSATS MEKVKVQEAV AQMDFQGEKL HRMYKERQGR

    FDRSVEKWRH FHYDMKVFNQ WLNEVEQFFK KTQNPENWEH AKYKWYLKEL

    QDGIGQRQAV VRTLNATGEE IIQQSSKTDV NILQEKLGSL SLRWHDICKE

    LAERRKRIEE QKNVLSEFQR DLNEFVLWLE EADNIAITPL GDEQQLKEQL

    EQVKLLAEEL PLRQGILKQL NETGGAVLVS APIRPEEQDK LEKKLKQTNL

    QWIKVSRALP EKQGELEVHL KDFRQLEEQL DHLLLWLSPI RNQLEIYNQP

    SQAGPFDIKE IEVTVHGKQA DVERLLSKGQ HLYKEKPSTQ PVKRKLEDLR

    SEWEAVNHLL RELRTKQPDR APGLSTTGAS ASQTVTLVTQ SVVTKETVIS

    KLEMPSSLLL EVPALADFNR AWTELTDWLS LLDRVIKSQR VMVGDLEDIN

    EMIIKQKATL QDLEQRRPQL EELITAAQNL KNKTSNQEAR TIITDRIERI

    QIQWDEVQEQ LQNRRQQLNE MLKDSTQWLE AKEEAEQVIG QVRGKLDSWK

    EGPHTVDAIQ KKITETKQLA KDLRQRQISV DVANDLALKL LRDYSADDTR

    KVHMITENIN TSWGNIHKRV SEQEAALEET HRLLQQFPLD LEKFLSWITE

    AETTANVLQD ASRKEKLLED SRGVRELMKP WQDLQGEIET HTDIYHNLDE

    NGQKILRSLE GSDEAPLLQR RLDNMNFKWS ELQKKSLNIR SHLEASSDQW

    KRLHLSLQEL LVWLQLKDDE LSRQAPIGGD FPAVQKQNDI HRAFKRELKT

    KEPVIMSTLE TVRIFLTEQP LEGLEKLYQE PRELPPEERA QNVTRLLRKQ

    AEEVNAEWDK LNLRSADWQR KIDEALERLQ ELQEAADELD LKLRQAEVIK

    GSWQPVGDLL IDSLQDHLEK VKALRGEIAP LKENVNRVND LAHQLTTLGI

    QLSPYNLSTL EDLNTRWRLL QVAVEDRVRQ LHEAHRDFGP ASQHFLSTSV

    QGPWERAISP NKVPYYINHE TQTTCWDHPK MTELYQSLAD LNNVRFSAYR

    TAMKLRRLQK ALCLDLLSLS AACDALDQHN LKQNDQPMDI LQIINCLTTI

    YDRLEQEHNN LVNVPLCVDM CLNWLLNVYD TGRTGRIRVL SFKTGIISLC

    KAHLEDKYRY LFKQVASSTG FCDQRRLGLL LHDSIQIPRQ LGEVASFGGS

    NIEPSVRSCF QFANNKPEIE AALFLDWMRL EPQSMVWLPV LHRVAAAETA

    KHQAKCNICK ECPIIGFRYR SLKHFNYDIC QSCFFSGRVA KGHKMHYPMV

    EYCTPTTSGE DVRDFAKVLK NKFRTKRYFA KHPRMGYLPV QTVLEGDNME

    TPVTLINFWP VDSAPASSPQ LSHDDTHSRI EHYASRLAEM ENSNGSYLND

    SISPNESIDD EHLLIQHYCQ SLNQDSPLSQ PRSPAQILIS LESEERGELE

    RILADLEEEN RNLQAEYDRL KQQHEHKGLS PLPSPPEMMP TSPQSPRDAE

    LIAEAKLLRQ HKGRLEARMQ ILEDHNKQLE SQLHRLRQLL EQPQAEAKVN

    GTTVSSPSTS LQRSDSSQPM LLRVVGSQTS ESMGEEDLLS PPQDTSTGLE

    EVMEQLNNSF PSSRGRNAPG KPMREDTM

  • UCSC uc009tri.2; mouse. [P11531-1];



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