Mouse Ddost ELISA Kit

Alternative name
Mouse DDOST 48 kDa subunit ELISA Kit;Mouse oligosaccharyl transferase 48 kDa subunit ELISA Kit;Mouse dolichyl-di-phosphooligosaccharide-protein glycotransferase ELISA Kit;Mouse dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit;
Catalog
E051203
species
Mouse
GeneDdost
SpecificityThis assay has high sensitivity and excellent specificity for detection of DDOST. No significant cross-reactivity or interference between DDOST and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of DDOST is typically less than 0.064ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseMouse Ddost ELISA Kit allows for the in vitro quantitative determination of Ddost , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDOST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DDOST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DDOST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DDOST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein information
Uniprot ID OST48_MOUSE
Uniprot AC O54734; Q8C4P7;
UniGene Mm.470295; Mm.7236;
GeneID 13200
KEGG mmu:13200;
Mouse Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein SEQUENCE
SEQUENCE 441 AA; 49028 MW; C1B9A0B94D2A1CAC CRC64;
MKMDPRLAVR AWPLCGLLLA VLGCVCASGP RTLVLLDNLN VRDTHSLFFR
SLKDRGFELT FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET
ISAFIDGGGS VLVAASSDIG DPLRELGSEC GIEFDEEKTA VIDHHNYDVS
DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVAD PDNPLVLDIL
TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFS
DAFFNSAVQK ATPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR
VGEMAPPNAY TVTDLVEYSI IIEQLSNGKW VPFDGDDIQL EFVRIDPFVR
TFLKRKGGKY SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH
TQYERFIPSA YPYYASAFSM MAGLFIFSIV FLHMKEKEKS D
UCSC uc008vkt.1; mouse.;

Category

Mouse Ddost ELISA Kit

Mouse DDOST 48 kDa subunit ELISA Kit;Mouse oligosaccharyl transferase 48 kDa subunit ELISA Kit;Mouse dolichyl-di-phosphooligosaccharide-protein glycotransferase ELISA Kit;Mouse dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit;