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Mouse Ece1 ELISA Kit

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  • Alternative name

    Mouse ECE-1 ELISA Kit;Mouse AW322500 ELISA Kit;Mouse ECE-1a ELISA Kit;Mouse ECE-1b ELISA Kit;Mouse ECE-1d ELISA Kit;Mouse endothelin converting enzyme 1 ELISA Kit;Mouse endothelin-converting enzyme 1 ELISA Kit;Mouse endothelin-converting enzyme-1a ELISA Kit;Mouse endothelin-converting enzyme-1b ELISA Kit;Mouse endothelin-converting enzyme-1d ELISA Kit;
  • Catalog
    E051524
  • species
    Mouse
  • GeneEce1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ECE1. No significant cross-reactivity or interference between Mouse ECE1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse ECE1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity9.375pg/mL
  • Intended UseMouse Ece1 ELISA Kit allows for the in vitro quantitative determination of Ece1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ECE1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECE1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ECE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ECE1. You can calculate the concentration of Mouse ECE1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Endothelin-converting enzyme 1 Protein information
  • Uniprot ID ECE1_MOUSE
  • Uniprot AC Q4PZA2; B1AXF9; Q4PZ99; Q4PZA1; Q6P9Q9;
  • UniGene Mm.401062; Mm.483336;
  • GeneID 230857
  • KEGG mmu:230857;
  • Mouse Endothelin-converting enzyme 1 Protein SEQUENCE

  • SEQUENCE 769 AA; 87073 MW; 58214938A0F2760A CRC64;

    MRTVWSPLAA ALAALGMSTY KRATLDEEDL VDSLSEGDVY PNGLQVNFRS

    SRSGQRCWAA RTSVEKRLVV LVTLLAAGLV ACLAALGIQY QTRTPPVCLT

    EACVSVTSSI LNSMDPTVDP CQDFFSYACG GWIKANPVPD GHSRWGTFSN

    LWEHNQAVIK HLLENATASV SEAERKAQVY YRACMNETRI EELRAKPLME

    LIEKLGGWNI TGPWAKDNFQ DTLQVVTAHY RTSPFFSVYV SADSKNSNSN

    VIQVDQSGLG LPSRDYYLNK TENEKVLTGY LNYMVQLGKL LGGGDEDAIR

    PQMQQILDFE TALANITIPQ EKRRDEELIY HKVTAAELQT LAPAINWLPF

    LNTIFYPVEI NESEPIVVYD KEYLRQVSTL INNTDKCLLN NYMMWNLVRK

    TSSFLDQRFQ DADEKFMEVM YGTKKTCIPR WKFCVSDTEN NLGFALGPMF

    VKATFAEDSK NIASEIIMEI KKAFEESLST LKWMDEETRR SAKEKADAIY

    NMIGYPNFIM DPKELDKVFN DYTAVPDLYF ENAMRFFNFS WRVTADQLRK

    APNRDQWSMT PPMVNAYYSP TKNEIVFPAG ILQAPFYTRS SPNALNFGGI

    GVVVGHELTH AFDDQGREYD KDGNLRPWWK NSSVEAFKQQ TECMVQQYSN

    YSVNGEPVNG RHTLGENIAD NGGLKAAYRA YQNWVKKNGA EQTLPTLGLT

    SNQLFFLGFA QVWCSVRTPE SSHEGLITDP HSPSRFRVIG SLSNSKEFSE

    HFRCPPGSPM NPHHKCEVW

  • UCSC uc008vjv.1; mouse. [Q4PZA2-2];



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