Mouse Eed ELISA Kit

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  • Alternative name

    Mouse l(7)5Rn ELISA Kit;Mouse l7Rn5 ELISA Kit;Mouse lusk ELISA Kit;Mouse embryonic ectoderm development ELISA Kit;Mouse polycomb protein EED ELISA Kit;Mouse lethal, Chr 7, Rinchik 5 ELISA Kit;

  • Catalog
    E051569
  • species
    Mouse
  • GeneEed
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse EED. No significant cross-reactivity or interference between Mouse EED and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse EED and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Eed ELISA Kit allows for the in vitro quantitative determination of Eed , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EED in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EED. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse EED and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EED, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse EED. You can calculate the concentration of Mouse EED in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Polycomb protein EED Protein information
  • Uniprot ID EED_MOUSE
  • Uniprot AC Q921E6; P97462;
  • UniGene Mm.380914;
  • GeneID 13626
  • KEGG mmu:13626;
  • Mouse Polycomb protein EED Protein SEQUENCE
  • SEQUENCE 441 AA; 50198 MW; D2E0A5BA27C0499A CRC64;

    MSEREVSTAP AGTDMPAAKK QKLSSDENSN PDLSGDENDD AVSIESGTNT

    ERPDTPTNTP NAPGRKSWGK GKWKSKKCKY SFKCVNSLKE DHNQPLFGVQ

    FNWHSKEGDP LVFATVGSNR VTLYECHSQG EIRLLQSYVD ADADENFYTC

    AWTYDSNTSH PLLAVAGSRG IIRIINPITM QCIKHYVGHG NAINELKFHP

    RDPNLLLSVS KDHALRLWNI QTDTLVAIFG GVEGHRDEVL SADYDLLGEK

    IMSCGMDHSL KLWRINSKRM MNAIKESYDY NPNKTNRPFI SQKIHFPDFS

    TRDIHRNYVD CVRWLGDLIL SKSCENAIVC WKPGKMEDDI DKIKPSESNV

    TILGRFDYSQ CDIWYMRFSM DFWQKMLALG NQVGKLYVWD LEVEDPHKAK

    CTTLTHHKCG AAIRQTSFSR DSSILIAVCD DASIWRWDRL R

  • UCSC uc009igk.2; mouse. [Q921E6-1];



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