Mouse Edn1 ELISA Kit

Alternative name
Mouse ET-1 ELISA Kit;Mouse preproendothelin-1 ELISA Kit;Mouse PPET1 ELISA Kit;Mouse preproET ELISA Kit;Mouse endothelin 1 ELISA Kit;Mouse endothelin-1 ELISA Kit;
Catalog
E051826
species
Mouse
GeneEdn1
SpecificityNatural and recombinant mouse Endothelin
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.5pg/ml
Intended UseMouse Edn1 ELISA Kit allows for the in vitro quantitative determination of Edn1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endothelin has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endothelin amount of sample captured in plate. Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
Mouse Big endothelin-1 Protein information
Uniprot ID EDN1_MOUSE
Uniprot AC P22387; P70203; P97338;
UniGene Mm.14543;
GeneID 13614
KEGG mmu:13614;
Mouse Big endothelin-1 Protein SEQUENCE
SEQUENCE 202 AA; 22809 MW; 3A0C1E1DFA797411 CRC64;
MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS
KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP
NKATDQAVRC QCAHQKDKKC WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS
KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS FRVAKLKAEL YRDQKLTHNR
AH
UCSC uc007qfl.2; mouse.;

Category

Mouse Edn1 ELISA Kit

Mouse ET-1 ELISA Kit;Mouse preproendothelin-1 ELISA Kit;Mouse PPET1 ELISA Kit;Mouse preproET ELISA Kit;Mouse endothelin 1 ELISA Kit;Mouse endothelin-1 ELISA Kit;