Mouse En2 ELISA Kit

Alternative name
Mouse homeobox protein en-2 ELISA Kit;Mouse mo-En-2 ELISA Kit;Mouse En-2 ELISA Kit;Mouse BB131122 ELISA Kit;Mouse engrailed 2 ELISA Kit;Mouse homeobox protein engrailed-2 ELISA Kit;
Catalog
E051867
species
Mouse
GeneEn2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse En2 ELISA Kit allows for the in vitro quantitative determination of En2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the Assay: EN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EN2 antibody and an EN2-HRP conjugate. The assay sample and buffer are incubated together with EN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EN2 concentration since EN2 from samples and EN2-HRP conjugate compete for the anti-EN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by EN2 from the sample, fewer sites are left to bind EN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EN2 concentration in each sample is interpolated from this standard curve.
Mouse Homeobox protein engrailed-2 Protein information
Uniprot ID HME2_MOUSE
Uniprot AC P09066;
UniGene Mm.4298;
GeneID 13799
KEGG mmu:13799;
Mouse Homeobox protein engrailed-2 Protein SEQUENCE
SEQUENCE 324 AA; 33817 MW; D1D9D602A1225A53 CRC64;
MEEKDSKPSE TAAEAQRQPE PSSGGGSGGG SSPSDSDTGR RRALMLPEVL
QAPGNHQHPH RITNFFIDNI LRPEFGRRKD AGTCCAGAGG ARGGEGGAGT
TEGGGGGAGG AEQLLGARES RPNPACAPSA GGTLSAAAGD PAVDGEGGSK
TLSLHGGAKK PGDPGGSLDG VLKARGLGGG DLSVSSDSDS SQASATLGAQ
PMLWPAWVYC TRYSDRPSSG PRSRKPKKKN PNKEDKRPRT AFTAEQLQRL
KAEFQTNRYL TEQRRQSLAQ ELSLNESQIK IWFQNKRAKI KKATGNKNTL
AVHLMAQGLY NHSTTAKEGK SDSE
UCSC uc008wtv.1; mouse.;

Category

Mouse En2 ELISA Kit

Mouse homeobox protein en-2 ELISA Kit;Mouse mo-En-2 ELISA Kit;Mouse En-2 ELISA Kit;Mouse BB131122 ELISA Kit;Mouse engrailed 2 ELISA Kit;Mouse homeobox protein engrailed-2 ELISA Kit;