Mouse Eif2ak3 ELISA Kit

Alternative name
Mouse PRKR-like endoplasmic reticulum kinase ELISA Kit;Mouse pancreatic eIF2-alpha kinase ELISA Kit;Mouse Pek ELISA Kit;Mouse Perk ELISA Kit;Mouse eukaryotic translation initiation factor 2 alpha kinase 3 ELISA Kit;Mouse eukaryotic translation initiation factor 2-alpha kinase 3 ELISA Kit;
Catalog
E052208
species
Mouse
GeneEif2ak3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.108ng/mL.
Intended UseMouse Eif2ak3 ELISA Kit allows for the in vitro quantitative determination of Eif2ak3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Eukaryotic translation initiation factor 2-alpha kinase 3 Protein information
Uniprot ID E2AK3_MOUSE
Uniprot AC Q9Z2B5; Q9CTK8; Q9CWT5;
UniGene Mm.247167;
GeneID 13666
KEGG mmu:13666;
Mouse Eukaryotic translation initiation factor 2-alpha kinase 3 Protein SEQUENCE
SEQUENCE 1114 AA; 124682 MW; 65A47D6C0DD2E046 CRC64;
MERATRPGPR ALLLLLFLLL GCAAGISAVA PARSLLAPAS ETVFGLGAAA
APTSAARVPA VATAEVTVED AEALPAAAGE PESRATEPDD DVELRPRGRS
LVIISTLDGR IAALDAENDG KKQWDLDVGS GSLVSSSLSK PEVFGNKMII
PSLDGDLFQW DRDRESMEAV PFTVESLLES SYKFGDDVVL VGGKSLITYG
LSAYSGKLRY ICSALGCRRW DSDEMEEEED ILLLQRTQKT VRAVGPRSGS
EKWNFSVGHF ELRYIPDMET RAGFIESTFK PGGNKEDSKI ISDVEEQEAT
MLDTVIKVSV ADWKVMAFSR KGGRLEWEYQ FCTPIASAWL VRDGKVIPIS
LFDDTSYTAS EEALGDEEDI VEAARGATEN SVYLGMYRGQ LYLQSSVRVS
EKFPTSPKAL ESVNGENAII PLPTIKWKPL IHSPSRTPVL VGSDEFDKCL
SNDKYSHEEY SNGALSILQY PYDNGYYLPY YKRERNKRST QITVRFLDSP
HYSKNIRKKD PILLLHWWKE IFGTILLCIV ATTFIVRRLF HPQPHRQRKE
SETQCQTESK YDSVSADVSD NSWNDMKYSG YVSRYLTDFE PIQCMGRGGF
GVVFEAKNKV DDCNYAIKRI RLPNRELARE KVMREVKALA KLEHPGIVRY
FNAWLETPPE KWQEEMDEIW LKDESTDWPL SSPSPMDAPS VKIRRMDPFS
TKEQIEVIAP SPERSRSFSV GISCGQTSSS ESQFSPLEFS GTDCGDNSDS
ADAAYNLQDS CLTDCEDVED GTVDGNDEGH SFELCPSEAS PYTRSREGTS
SSIVFEDSGC GNASSKEEPR GNRLHDGNHY VNKLTDLKCS SSRSSSEATT
LSTSPTRPTT LSLDFTKNTV GQLQPSSPKV YLYIQMQLCR KENLKDWMNR
RCSLEDREHG VCLHIFLQIA EAVEFLHSKG LMHRDLKPSN IFFTMDDVVK
VGDFGLVTAM DQDEEEQTVL TPMPAYATHT GQVGTKLYMS PEQIHGNNYS
HKVDIFSLGL ILFELLYPFS TQMERVRILT DVRNLKFPLL FTQKYPQEHM
MVQDMLSPSP TERPEATDII ENAIFENLEF PGKTVLRQRS RSMSSSGTKH
SRQPSCSYSP LPGN
UCSC uc009cgc.1; mouse.;

Category

Mouse Eif2ak3 ELISA Kit

Mouse PRKR-like endoplasmic reticulum kinase ELISA Kit;Mouse pancreatic eIF2-alpha kinase ELISA Kit;Mouse Pek ELISA Kit;Mouse Perk ELISA Kit;Mouse eukaryotic translation initiation factor 2 alpha kinase 3 ELISA Kit;Mouse eukaryotic translation initiation factor 2-alpha kinase 3 ELISA Kit;