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Mouse Eif2a ELISA Kit

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  • Alternative name

    Mouse eIF-2A ELISA Kit;Mouse D3Ertd194e ELISA Kit;Mouse D030048D22 ELISA Kit;Mouse eukaryotic translation initiation factor 2A ELISA Kit;
  • Catalog
    E052215
  • species
    Mouse
  • GeneEif2a
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of EIF2A. No significant cross-reactivity or interference between EIF2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EIF2A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Eif2a ELISA Kit allows for the in vitro quantitative determination of Eif2a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: EIF2A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EIF2A. Standards or samples are then added to the microtiter plate wells and EIF2A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EIF2A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EIF2A are added to each well to "sandwich" the EIF2A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EIF2A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF2A concentration in each sample is interpolated from this standard curve.
  • Mouse Eukaryotic translation initiation factor 2A, N-terminally processed Protein information
  • Uniprot ID EIF2A_MOUSE
  • Uniprot AC Q8BJW6; Q05C11; Q640P8;
  • UniGene Mm.21617;
  • GeneID 229317
  • KEGG mmu:229317;
  • Mouse Eukaryotic translation initiation factor 2A, N-terminally processed Protein SEQUENCE

  • SEQUENCE 581 AA; 64403 MW; D485C05ACE5D0908 CRC64;

    MAPSTPLLTV RGSEGLYMVN GPPHFTESTV LPRESGRNCK VYTFSKDGTL

    FAWSNGEKVN IINVANKGLL HSFDLPKAVC LEFSPNNTVL ATWQPYTTSK

    DGTAGTPNLQ LYDMKTGACL KSFIQKKMQN WCPSWSDDEI ICARNVNNEV

    HFFENNNFNT IANKLHLQKV NDFNLSPGTQ PYKVAVYVPG SKGAPSFVRL

    YQYPNFAGPQ AALANKSFFK ADKVTMLWNK KATAVLVIAS TEVDKTGASY

    YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT

    VFNLKCDPVF DFGTGPRNAA FYSPHGHILV LAGFGNLRGQ MEVWDVKNYK

    LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSLLHKY

    DVPSNGELWQ VSWQPFLDGI FPAKTIKYQA VPSEVPSEEP KVATAYRPPA

    LRNKPVTNSK LHEEEPPQNM KPHPGSDKPL SKTALKNQRK HEAKKAAKQE

    ARSDAAPTPV PQSAPRNTVT QSASGDPEVD KKIKNLKKKL KAIEQLKEQA

    AAGKQLEKNQ LEKIQKETAL LQELEDLELG V

  • UCSC uc008phv.2; mouse. [Q8BJW6-2];



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