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Human BCAT1 ELISA Kit

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  • Alternative name

    Human BCAT(c) ELISA Kit;Human Protein ECA39 ELISA Kit;Human BCT1 ELISA Kit;Human ECA39 ELISA Kit;Human BCATC ELISA Kit;Human MECA39 ELISA Kit;Human PNAS121 ELISA Kit;Human PP18 ELISA Kit;Human branched chain amino acid transaminase 1 ELISA Kit;Human branched-chain-amino-acid aminotransferase, cytosolic ELISA Kit;Human branched chain amino-acid transaminase 1, cytosolic ELISA Kit;Human branched chain aminotransferase 1, cytosolic ELISA Kit;Human placental protein 18 ELISA Kit;
  • Catalog
    E005263
  • species
    Human
  • GeneBCAT1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BCAT1. No significant cross-reactivity or interference between BCAT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCAT1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman BCAT1 ELISA Kit allows for the in vitro quantitative determination of BCAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This BCAT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BCAT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: BCAT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCAT1 antibody and an BCAT1-HRP conjugate. The assay sample and buffer are incubated together with BCAT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCAT1 concentration since BCAT1 from samples and BCAT1-HRP conjugate compete for the anti-BCAT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCAT1 from the sample, fewer sites are left to bind BCAT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCAT1 concentration in each sample is interpolated from this standard curve.
  • Human Branched-chain-amino-acid aminotransferase, cytosolic Protein information
  • Uniprot ID BCAT1_HUMAN
  • Uniprot AC P54687; B3KY27; B7Z2M5; B7Z5L0; F5H5E4; Q68DQ7; Q96MY9;
  • UniGene Hs.438993;
  • GeneID 586
  • KEGG hsa:586;
  • Human Branched-chain-amino-acid aminotransferase, cytosolic Protein SEQUENCE

  • SEQUENCE 386 AA; 42966 MW; A43535303A702512 CRC64;

    MKDCSNGCSA ECTGEGGSKE VVGTFKAKDL IVTPATILKE KPDPNNLVFG

    TVFTDHMLTV EWSSEFGWEK PHIKPLQNLS LHPGSSALHY AVELFEGLKA

    FRGVDNKIRL FQPNLNMDRM YRSAVRATLP VFDKEELLEC IQQLVKLDQE

    WVPYSTSASL YIRPTFIGTE PSLGVKKPTK ALLFVLLSPV GPYFSSGTFN

    PVSLWANPKY VRAWKGGTGD CKMGGNYGSS LFAQCEAVDN GCQQVLWLYG

    EDHQITEVGT MNLFLYWINE DGEEELATPP LDGIILPGVT RRCILDLAHQ

    WGEFKVSERY LTMDDLTTAL EGNRVREMFG SGTACVVCPV SDILYKGETI

    HIPTMENGPK LASRILSKLT DIQYGREESD WTIVLS

  • UCSC uc001rgc.4; human. [P54687-1];



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