SpecificityThis assay has high sensitivity and excellent specificity for detection of FABP7. No significant cross-reactivity or interference between FABP7 and analogues was observed.
Intended UseMouse Fabp7 ELISA Kit allows for the in vitro quantitative determination of Fabp7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FABP7 in mouse serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to FABP7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FABP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FABP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FABP7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Fatty acid-binding protein, brain Protein information
Uniprot ID
FABP7_MOUSE
Uniprot AC
P51880; Q4FJK4;
UniGene
Mm.3644;
GeneID
12140
KEGG
mmu:12140;
Mouse Fatty acid-binding protein, brain Protein SEQUENCE