Mouse Faah ELISA Kit

Alternative name
Mouse anandamide amidohydrolase 1 ELISA Kit;Mouse oleamide hydrolase 1 ELISA Kit;Mouse Faah1 ELISA Kit;Mouse AW412498 ELISA Kit;Mouse fatty acid amide hydrolase ELISA Kit;Mouse fatty-acid amide hydrolase 1 ELISA Kit;Mouse amide hydrolase ELISA Kit;Mouse fatty acid amide hydrolase protein ELISA Kit;
Catalog
E052791
species
Mouse
GeneFaah
SpecificityThis assay has high sensitivity and excellent specificity for detection of FAAH. No significant cross-reactivity or interference between FAAH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FAAH and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Faah ELISA Kit allows for the in vitro quantitative determination of Faah , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: FAAH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FAAH antibody and an FAAH-HRP conjugate. The assay sample and buffer are incubated together with FAAH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAAH concentration since FAAH from samples and FAAH-HRP conjugate compete for the anti-FAAH antibody binding site. Since the number of sites is limited, as more sites are occupied by FAAH from the sample, fewer sites are left to bind FAAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAAH concentration in each sample is interpolated from this standard curve.
Mouse Fatty-acid amide hydrolase 1 Protein information
Uniprot ID FAAH1_MOUSE
Uniprot AC O08914; Q922S0; Q9DBF5;
UniGene Mm.256025;
GeneID 14073
KEGG mmu:14073;
Mouse Fatty-acid amide hydrolase 1 Protein SEQUENCE
SEQUENCE 579 AA; 63221 MW; 11B2DF6CBC110015 CRC64;
MVLSEVWTAL SGLSGVCLAC SLLSAAVVLR WTRSQTARGA VTRARQKQRA
GLETMDKAVQ RFRLQNPDLD SEALLALPLL QLVQKLQSGE LSPEAVLFTY
LGKAWEVNKG TNCVTSYLTD CETQLSQAPR QGLLYGVPVS LKECFSYKGH
ASTLGLSLNE GVTSESDCVV VQVLKLQGAV PFVHTNVPQS MLSYDCSNPL
FGQTMNPWKP SKSPGGSSGG EGALIGSGGS PLGLGTDIGG SIRFPSAFCG
ICGLKPTGNR LSKSGLKSCV YGQTAVQLSV GPMARDVDSL ALCMKALLCE
DLFRLDSTIP PLPFREEIYR SSRPLRVGYY ETDNYTMPTP AMRRAVMETK
QSLEAAGHTL VPFLPNNIPY ALEVLSAGGL FSDGGCSFLQ NFKGDFVDPC
LGDLVLVLKL PRWFKKLLSF LLKPLFPRLA AFLNSMCPRS AEKLWELQHE
IEMYRQSVIA QWKAMNLDVV LTPMLGPALD LNTPGRATGA ISYTVLYNCL
DFPAGVVPVT TVTAEDDAQM EHYKGYFGDM WDNILKKGMK KGIGLPVAVQ
CVALPWQEEL CLRFMREVER LMTPEKRPS
UCSC uc008ufs.2; mouse.;

Category

Mouse Faah ELISA Kit

Mouse anandamide amidohydrolase 1 ELISA Kit;Mouse oleamide hydrolase 1 ELISA Kit;Mouse Faah1 ELISA Kit;Mouse AW412498 ELISA Kit;Mouse fatty acid amide hydrolase ELISA Kit;Mouse fatty-acid amide hydrolase 1 ELISA Kit;Mouse amide hydrolase ELISA Kit;Mouse fatty acid amide hydrolase protein ELISA Kit;