Mouse Fli1 ELISA Kit

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  • Alternative name

    Mouse retroviral integration site protein Fli-1 ELISA Kit;Mouse Fli-1 ELISA Kit;Mouse EWSR2 ELISA Kit;Mouse SIC-1 ELISA Kit;Mouse Sic1 ELISA Kit;Mouse Friend leukemia integration 1 ELISA Kit;Mouse Friend leukemia integration 1 transcription factor ELISA Kit;

  • Catalog
    E053223
  • species
    Mouse
  • GeneFli1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse FLI1. No significant cross-reactivity or interference between Mouse FLI1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse FLI1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Fli1 ELISA Kit allows for the in vitro quantitative determination of Fli1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FLI1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FLI1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse FLI1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FLI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse FLI1. You can calculate the concentration of Mouse FLI1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Friend leukemia integration 1 transcription factor Protein information
  • Uniprot ID FLI1_MOUSE
  • Uniprot AC P26323;
  • UniGene Mm.258908;
  • GeneID 14247
  • KEGG mmu:14247;
  • Mouse Friend leukemia integration 1 transcription factor Protein SEQUENCE
  • SEQUENCE 452 AA; 51002 MW; 1F9A06C6893FE2AB CRC64;

    MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP

    LPPQQEWINQ PVRVNVKREY DHMNGSRESP VDCSVSKCNK LVGGGEANPM

    NYNSYMDEKN GPPPPNMTTN ERRVIVPADP TLWTQEHVRQ WLEWAIKEYG

    LMEIDTSFFQ NMDGKELCKM NKEDFLRATS AYNTEVLLSH LSYLRESSLL

    AYNTTSHTDQ SSRLNVKEDP SYDSVRRGAW NNNMNSGLNK SPLLGGSQTM

    GKNTEQRPQP DPYQILGPTS SRLANPGSGQ IQLWQFLLEL LSDSANASCI

    TWEGTNGEFK MTDPDEVARR WGERKSKPNM NYDKLSRALR YYYDKNIMTK

    VHGKRYAYKF DFHGIAQALQ PHPTETSMYK YPSDISYMPS YHAHQQKVNF

    VPSHPSSMPV TSSSFFGAAS QYWTSPTAGI YPNPSVPRHP NTHVPSHLGS

    YY

  • UCSC uc009orz.2; mouse.;



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