Mouse Fos ELISA Kit

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  • Alternative name

    Mouse cellular oncogene fos ELISA Kit;Mouse D12Rfj1 ELISA Kit;Mouse c-fos ELISA Kit;Mouse cFos ELISA Kit;Mouse FBJ osteosarcoma oncogene ELISA Kit;Mouse proto-oncogene c-Fos ELISA Kit;Mouse proto-oncogene protein c-fos ELISA Kit;

  • Catalog
    E053394
  • species
    Mouse
  • GeneFos
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse FOS. No significant cross-reactivity or interference between Mouse FOS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse FOS and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Fos ELISA Kit allows for the in vitro quantitative determination of Fos , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FOS in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FOS. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse FOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse FOS. You can calculate the concentration of Mouse FOS in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Proto-oncogene c-Fos Protein information
  • Uniprot ID FOS_MOUSE
  • Uniprot AC P01101;
  • UniGene Mm.246513;
  • GeneID 14281
  • KEGG mmu:14281;
  • Mouse Proto-oncogene c-Fos Protein SEQUENCE
  • SEQUENCE 380 AA; 40838 MW; 475966265952B624 CRC64;

    MMFSGFNADY EASSSRCSSA SPAGDSLSYY HSPADSFSSM GSPVNTQDFC

    ADLSVSSANF IPTVTAISTS PDLQWLVQPT LVSSVAPSQT RAPHPYGLPT

    QSAGAYARAG MVKTVSGGRA QSIGRRGKVE QLSPEEEEKR RIRRERNKMA

    AAKCRNRRRE LTDTLQAETD QLEDEKSALQ TEIANLLKEK EKLEFILAAH

    RPACKIPDDL GFPEEMSVAS LDLTGGLPEA STPESEEAFT LPLLNDPEPK

    PSLEPVKSIS NVELKAEPFD DFLFPASSRP SGSETSRSVP DVDLSGSFYA

    ADWEPLHSNS LGMGPMVTEL EPLCTPVVTC TPGCTTYTSS FVFTYPEADS

    FPSCAAAHRK GSSSNEPSSD SLSSPTLLAL

  • UCSC uc007oha.2; mouse.;



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